Theknoloji ea Hybridoma e ka sebelisoa ho lokisa li-antibodies tsa monoclonal. Ka ho kopanya lisele tsa spleen ho tsoa ho litoeba tse entetsoeng le lisele tsa myeloma ho tsoa ho batho kapa litoeba, lisele tsa hybridoma lia thehoa 'me li ntša li-antibodies tse itseng. Li-antibodies tsa monoclonal tse hlahisitsoeng tse tsoang ho toeba li ka sebelisoa lipatlisisong tsa saense tse kang tlhahlobo ea immunosorbent e kopantsoeng le enzyme, radioimmunoassay, immunohistochemistry, flow cytometry, protein purification, jj. Lisampole tsa li-antibody tsa monoclonal li kenyelletsa ascites, hybridoma supernatant, jj. Sampole e fumanoeng ka ho kenya lisele tsa hybridoma ka har'a mpa ea liphoofolo le ho sebelisa tikoloho ea 'mele ea mpa ea phoofolo ho hlahisa li-antibodies e bitsoa ascites sample. Lisele tsa Hybridoma tse kenngoang ka har'a mpa ea liphoofolo li ka ata le ho ntša li-antibodies tsa monoclonal, tse ka bokellanang ka har'a ascites. Ka lebaka la monyetla oa litšila (liprotheine tsa liphoofolo, lihormone, jj.) li-antibodies tsena, ho hlokahala tlhoekiso. Ho latela mosebetsi oa ho ntša li-lymphocyte tsa B, li-antibodies tsa monoclonal hangata li ntšoa ka har'a sebaka sa temo ha ho lengoa lisele tsa hybridoma. Mokelikeli o nang le li-antibodies tsa monoclonal sebakeng sa tlhahiso ea limela o bitsoa hybridoma supernatant. Bohloeki ba li-antibodies tsa monoclonal sebakeng sa tlhahiso ea limela bo phahame haholo, empa tlhoekiso e eketsehileng ea hlokahala bakeng sa ho lokisoa ka bongata kaha li fumanoa ka tlhahiso ea in vitro.

Ho hlwekisa di-antibody ho ka arola di-antibodies tse hlwekileng haholo ho tswa ho di-complexes. Hona jwale, ho hlwekisa di-antibody affinity le karohano le tlhoekiso ya di-antibody ke mekgwa e sebediswang haholo ya ho hlwekisa di-antibody, haholoholo ho kenyeletswa le tlhoekiso ya protheine A/G, chromatography ya phapanyetsano ya ion, ho sefa di-gel, precipitation le hydrophobic interaction chromatography. Har'a tsona, tshebeletso ya tlhoekiso ya di-antibody ya protheine A/G e tumme haholo ditshebedisong tse fapaneng tsa tlhoekiso ya di-antibody. Tshebetso ya Alpha Lifetech ho lokisa di-antibodies tsa monoclonal tse itshetlehileng hodima theknoloji ya hybridoma e bontshitswe ho Setšoantšo sa 1.

Setšoantšo sa 1. Setšoantšo sa ts'ebetso bakeng sa ho lokisa li-antibodies tsa monoclonal tse thehiloeng theknolojing ea hybridoma.