Phage Display Antibody Development Platform

Alpha Lifetech, e nang le boiphihlelo bo pharaletseng ba projeke ea ho sibolla li-antibody, e ka fana ka lits'ebeletso tse ikhethileng tsa li-monoclonal le polyclonal antibody bakeng sa mefuta e mengata, hammoho le lits'ebeletso tsa kaho ea laebrari ea antibody le lits'ebeletso tsa tlhahlobo. Alpha Lifetech e ka fa bareki li-antibody tse netefalitsoeng tsa boleng le lihlahisoa tsa anti-anti-recombinant, hammoho le lits'ebeletso tse tsamaellanang, ho lokisa li-antibodies tse sebetsang hantle, tse tobileng haholo le tse tsitsitseng. Ka ho sebelisa liforomo tse felletseng tsa li-antibody le boenjineri ba li-antibody, re fana ka lits'ebeletso tse koahelang karolo e ka holimo le e tlase ea tlhahiso ea li-antibody, ho kenyeletsoa litšebeletso tsa tekheniki tse kang ho lokisa li-antibody, tlhoekiso, tatellano ea li-antibody, le netefatso.

Alpha Lifetech e na le sethala sa ho sibolla batho ba seng ba hōlile se fanang ka litšebeletso tse ngata tse kenyeletsang ho itšireletsa ha liphoofolo, ho haha laebrari ea li-antibody le ho hlahloba, ho latellana ha li-antibody, ho hloekisa antibody, ho netefatsa antibody, le ho ngola li-antibody ho latela theknoloji ea hybridoma, thekenoloji e le 'ngoe ea B cell, theknoloji ea phage display, le tse ling. Alpha Lifetech e na le mohala o tloaelehileng oa tlhahiso, 'me ntle le lits'ebeletso tsa ho lokisa li-antibody, litšebeletso tse tšehetsang tse kang antibody humanization, antibody affinity maturation, moralo le nts'etsopele ea lithethefatsi tsa ADC, le moralo o latelang oa CAR-T le ona o ka fanoa. Ka nako e ts'oanang, Alpha Lifetech e hahile laebrari ea antibody e thehiloeng ho M13, T4, le T7 phage display platforms, e nang le bokhoni ba ho boloka ho fihla ho 10 ^ 8-10 ^ 9. Sekhahla se setle, sekhahla sa ho kenya, le mefuta-futa ea laebrari kaofela li ka fihla ho feta 90%.

Phage e bonts'a Theknoloji

Tlhahiso ea li-antibodies tsa monoclonal e qalile ho sebelisoa ho sebelisoa theknoloji ea hybridoma monoclonal antibody ho hlahisa mouse hybridoma monoclonal antibody. Haholo-holo, ka ho kopanya lisele tsa spleen ho tloha ho litoeba tse entetsoeng le lisele tsa myeloma tse tsoang ho batho kapa litoeba, lisele tsa hybridoma li thehoa, tse hlahisang li-antibodies tsa hybridoma monoclonal antibody. Ka phetoho ea motho ea li-antibodies tsa mouse le phetoho ea liphatsa tsa lefutso tsa li-antibodies ho li fa sebaka sa kamehla sa immunoglobulin ea motho, immunogenicity ea bona e ka fokotseha. Monoclonal antibody engineering e sebelisoa haholo lits'ebetsong tsa biomedical le tsa bongaka.

Alpha Lifetech e ka fana ka lits'ebeletso tsa boenjineri tsa antibody tse emisang motho a le mong ho tloha ho moralo oa protocol oa lipatlisiso ho isa ho ho sibolloa ha li-antibody drug conjugates (ADCs) le netefatso ea liphoofolo. Li-antibody drug conjugates (ADCs) li ka isa meriana ea chemotherapy liseleng tsa mofetše. Kamora ho ikamahanya le lipheo tse ikhethileng tse hlahisitsoeng liseleng tsa mofetše, ADC e ntša lithethefatsi tsa cytotoxic liseleng tsa mofetše. Alpha Lifetech e ka boela ea fa bareki lits'ebeletso tse felletseng tsa kholo ea antibody. Ka ntlafatso e tsoetseng pele ea phetoho le theknoloji ea ho hlahloba phage e phahameng, li-antibodies tse nang le kamano e itseng li qala ho hlahlojoa, ebe liphetoho tsa amino acid li hlahisoa ho hlahisa mefuta e sa tšoaneng. Ka mor'a moo, li-antibodies tse nang le kamano e phahameng li ile tsa hlahlojoa le ho hlahlojoa ho sebelisoa mekhoa ea ho hlahloba. Kamora mekhahlelo e mengata ea ntlafatso le tlhahlobo ea sebopeho, li-antibodies tse nang le kamano e phahameng le tse ikhethang tse matla li ile tsa qetella li fumanoe.

Alpha Lifetech e ka aha mefuta e fapaneng ea lilaebrari tse bonts'ang li-antibody phage, ho kenyeletsoa lilaebrari tsa 'mele oa ho itšireletsa mafung, lilaeborari tsa matsoalloa, lilaebrari tsa maiketsetso le lilaebrari tsa maiketsetso. Ho ipapisitsoe le bokhoni bo phahameng ba lilaebrari tsa li-antibody, li-antibodies tse khethehileng li ka fumanoa. Li-vector tse ngata tsa phagemid tse kang pMECS, pComb3X, le pCANTAB 5E li ka fanoa, hammoho le mefuta e kang TG1 E. coli, XL1 Blue, le ER2738. Ntle le bokhoni ba laeborari ea ho fihla ho 10^9, sekhahla se kenngoeng sa likhechana tsa laebrari le sona se holimo, se theha maemo a lekaneng a ho hlahloba li-antibodies tse khotsofatsang bareki. Chate ea phallo ea ts'ebeletso ea ho lokisa ea Alpha Lifetech ea antibody e ipapisitseng le theknoloji ea ponts'o ea phage e bonts'itsoe ho Setšoantšo sa 1.

Setšoantšo sa 1 Setšoantšo sa ts'ebetso ea ho lokisetsa li-antibodies tsa monoclonal tse thehiloeng ho theknoloji ea pontšo ea phage.

Phage Display Antibody Production Workflow

Mehato	Litaba tsa Tšebeletso	Lenane la nako
Mohato oa Pele: Ente ea liphoofolo	(1) Ho entoa ka liphoofolo ka makhetlo a 4, ente ea booster 1 lethal dose, kakaretso ea litekanyetso tse 5 tse entetsoeng. (2) Serum e mpe pele ho entoa e bokelloa, 'me ELISA e ile ea etsoa ka tekanyo ea bone ho lemoha serum titer. (3) Haeba serum antibody titer ea tekanyo ea bone e kopana le litlhoko, tekanyo e le 'ngoe e eketsehileng ea ente e tla tsamaisoa matsatsi a 7 pele ho pokello ea mali. Haeba e sa fihlelle litlhokahalo, ente e tloaelehileng e tla tsoela pele. (4) Potency e tšoanelehang, pokello ea mali le ho arohana ha monocyte	Libeke tse 10
Mohato oa 2: Tokisetso ea cDNA	(1) PBMC Total RNA Extraction (RNA Extraction Kit) (2) Boitšoaro bo phahameng ba RT-PCR ba cDNA (reverse transcription kit)	1 letsatsi
Mohato oa 3: Kaho ea Laeborari ea Li-Antibody	(1) Ka ho sebelisa cDNA e le template, liphatsa tsa lefutso li ile tsa matlafatsoa ke likarolo tse peli tsa PCR. (2) Kaho ea Phage le phetoho: gene splicing phagemid vector, electroporation transformation ea TG1 host bacteria, kaho ea laebrari ea antibody. (3) Boitsebiso: Khetha ka mokhoa o sa reroang li-clone tse 24, sekhahla sa boitsebiso ba PCR + sekhahla sa ho kenya. (4) Thuso ea ho lokisa phage: M13 phage amplification + hloekisa. (5) Phage e bonts'a laebrari ea pholoso	Libeke tse 3-4
Mohato oa 4: Tlhahlobo ea Laebrari ea Antibody (mekhahlelo e 3)	(1) Tlhahlobo ea kamehla ea 3-round screening (ts'ebetso e tiileng ea mohato): khatello ea khatello ea ho tlosa li-antibodies tse sa tsitsang ho fihlela moo ho ka khonehang. (2) Single clone amplification bacteriophage select+IPTG induced expression+ELISA ho lemoha ha li-clone tse ntle. (3) Li-clone tsohle tse ntle li ile tsa khethoa bakeng sa tatellano ea liphatsa tsa lefutso.	Libeke tse 4-5

Molemo Oa Phage Bontša Theknoloji

Alpha Lifetech e na le boiphihlelo bo bongata lefapheng la nts'etsopele ea li-antibody. Ho theosa le lilemo, Alpha Lifetech e thehile sethala se felletseng sa nts'etsopele ea li-antibody.

Litšebeletso tsa Tšehetso

Re ka fana ka lits'ebeletso tse fapaneng tsa kaho ea laeborari ea 'mele ea ho itšireletsa mafung le lits'ebeletso tsa tlhahlobo ea laeborari ea antibody ho latela litlhoko tsa bareki

Lipheo tse ngata

Litšebeletso tse ngata tsa ho sibolla li-antibody lia fumaneha: liprotheine, li-peptide, limolek'hule tse nyane, livaerase, liprotheine tsa membrane, mRNA, jj.

Li-Vector tse ngata

Ts'ebeletso ea kaho ea laeborari e ikhethileng, re ka fana ka li-vector tse fapaneng tsa bacteriophage ho kenyelletsa PMECS, pComb3X, le pCANTAB 5E, 'me ra li fetola ho latela litlhoko tsa bareki.

Sethala se holileng

Bokhoni ba polokelo bo ka fihla ho 10 ^ 8-10 ^ 9, litefiso tsa ho kenya kaofela li ka holimo ho 90%, 'me kamano ea li-antibodies tse fumanoang ka ho hlahloba hangata li boemong ba nM pM.

TŠEBELETSO E Amanang

Maano a Mangata a Nts'etsopele ea Likokoana-hloko

Tšebeletso ea Ntlafatso ea Monoclonal Antibody

Hybridoma Technology Platform

Sethala sa ho Hlopha Lisele tsa B Single

Phage Display Antibody Development Platform

nts'etsopele ea antibody ea monoclonal-Alpha Lifetech

Tšebeletso ea Ntlafatso ea Monoclonal Antibody

Re ka fana ka lits'ebeletso tsa nts'etsopele ea boleng bo holimo, bohloeki bo holimo, le bo ikhethileng haholo, ho kenyeletsoa tlhahiso ea li-anti-monoclonal tsa mouse le li-anti-monoclonal tsa mmutla.

Hybridoma Technology Platform

Ho kenyelletsa lenaneo la ente, lits'ebeletso tsa ho lokisa li-antibody, tlhoekiso ea li-antibody, tatellano ea li-antibody tse phahameng, netefatso ea antibody, joalo-joalo.

Sethala sa ho Hlopha Lisele tsa B Single

Alpha Lifetech e na le melemo nakong ea tlhahlobo le ho fumana li-antibodies tsa boleng bo holimo. E ka fana ka moralo oa antigen, motsoako le phetoho, ho itšireletsa ha liphoofolo, tlhahlobo e le 'ngoe ea B cell enrichment, tatellano ea sele e le 'ngoe.