Sethala sa Ntlafatso ea Antibody sa Pontšo ea Phage

Alpha Lifetech, ka boiphihlelo ba eona bo boholo ba projeke ea ho sibolla li-antibody, e ka fana ka lits'ebeletso tsa li-antibody tsa monoclonal le polyclonal tse ikhethileng bakeng sa mefuta e mengata, hammoho le lits'ebeletso tsa kaho le tlhahlobo ea laeborari ea li-antibody. Alpha Lifetech e ka fa bareki lihlahisoa tsa li-antibody le li-antibody tse netefalitsoeng tsa boleng bo holimo, hammoho le lits'ebeletso tse tsamaellanang, ho lokisa li-antibody tse sebetsang hantle, tse ikhethileng haholo le tse tsitsitseng. Ka ho sebelisa li-platform tse felletseng tsa li-antibody le boenjiniere ba li-antibody, re fana ka lits'ebeletso tse akaretsang tlhahiso ea li-antibody e holimo le e tlase, ho kenyeletsoa lits'ebeletso tsa tekheniki tse kang ho lokisa li-antibody, tlhoekiso, tatellano ea li-antibody le netefatso.

Alpha Lifetech e na le sethala sa ho sibolla li-antibody tse hōlileng tsebong se fang bareki mefuta e fapaneng ea lits'ebeletso ho kenyeletsoa le ho itšireletsa mafung a liphoofolo, kaho le tlhahlobo ea laeborari ea li-antibody tsa phage ponts'o, tatellano ea li-antibody, tlhahiso ea li-antibody, tlhoekiso ea li-antibody, netefatso ea li-antibody, le ho ngola li-antibody ho latela theknoloji ea hybridoma, theknoloji ea sele e le 'ngoe ea B, theknoloji ea pontšo ea phage, le tse ling. Alpha Lifetech e na le mohala o tloaelehileng oa tlhahiso, 'me ntle le lits'ebeletso tsa ho lokisa li-antibody, lits'ebeletso tse tšehetsang tse kang ho etsa hore li-antibody li be motho, ho hola ha li-antibody, moralo le nts'etsopele ea lithethefatsi tsa ADC, le moralo oa tatellano o latelang oa CAR-T le tsona li ka fanoa. Ka nako e ts'oanang, Alpha Lifetech e hahile laeborari ea li-antibody e thehiloeng ho li-platform tsa theknoloji ea pontšo ea phage ea M13, T4, le T7, e nang le bokhoni ba polokelo bo fihlang ho 10^8-10^9. Sekhahla se setle, sekhahla sa ho kenya, le mefuta-futa ea laeborari kaofela li ka fihla ho feta 90%.

Theknoloji ea ponts'o ea phage

Tlhahiso ea li-antibodies tsa monoclonal e qalile ho lokisoa ho sebelisoa theknoloji ea li-antibody tsa monoclonal tsa hybridoma ho hlahisa li-antibody tsa monoclonal tsa mouse hybridoma. Haholo-holo, ka ho kopanya lisele tsa spleen ho tsoa ho litoeba tse entetsoeng ka lisele tsa myeloma ho tsoa ho batho kapa litoeba, lisele tsa hybridoma lia thehoa, tse ntšang li-antibody tsa monoclonal tse itseng tsa hybridoma. Ka phetoho ea li-antibodies tsa monoclonal tsa mouse tse entsoeng ka botho le phetoho ea boenjiniere ba liphatsa tsa lefutso ea li-antibodies ho li fa sebaka se sa feleng sa immunoglobulin ea motho, matla a tsona a ho itšireletsa a ka fokotseha. Boenjiniere ba li-antibody tsa monoclonal bo sebelisoa haholo lits'ebetsong tsa bongaka le tsa bongaka.

Alpha Lifetech e ka fana ka lits'ebeletso tsa boenjiniere ba antibody tse emisang ntho e le 'ngoe ho tloha moralo oa protocol ea lipatlisiso tsa pele ho kliniki ho ea ho ho sibolloa ha li-conjugates tsa antibody drug (ADCs) le netefatso ea liphoofolo. Li-conjugates tsa antibody drug (ADCs) li ka fana ka meriana ea chemotherapy liseleng tsa mofetše. Kamora ho tlamahana le lipheo tse itseng tse hlalositsoeng liseleng tsa mofetše, ADC e ntša meriana e chefo liseleng tsa mofetše. Alpha Lifetech e ka boela ea fa bareki lits'ebeletso tse felletseng tsa ho holisa antibody affinity. Ka ntlafatso e tsoetseng pele ea phetoho le theknoloji ea tlhahlobo ea phage e nang le throughput, li-antibodies tse nang le affinity e itseng li hlahlojoa pele, ebe liphetoho tsa amino acid li hlahisoa ho hlahisa mefuta e fapaneng. Ka mor'a moo, li-antibodies tse nang le affinity e phahameng li ile tsa hlahlojoa le ho hlahlojoa ho sebelisoa mekhoa ea tlhahlobo. Kamora mekhahlelo e mengata ea ntlafatso le tlhahlobo ea sebopeho, li-antibodies tse nang le affinity e phahameng le tse tobileng tse matla qetellong li ile tsa fumanoa.

Alpha Lifetech e ka haha mefuta e fapaneng ea lilaebrari tsa ponts'o ea antibody phage, ho kenyeletsoa lilaebrari tsa 'mele, lilaebrari tsa tlhaho, lilaebrari tsa semi synthetic, le lilaebrari tsa maiketsetso. Ho ipapisitsoe le bokhoni bo phahameng ba lilaebrari tsa antibody, li-antibodies tse ikhethileng haholo li ka fumanoa. Li-vector tse ngata tsa phagemid tse kang pMECS, pComb3X, le pCANTAB 5E li ka fanoa, hammoho le mefuta e kang TG1 E. coli, XL1 Blue, le ER2738. Ntle le bokhoni ba laebrari ba ho fihla ho 10^9, sekhahla sa ho kenngoa ha likarolo tse reriloeng sa laebrari le sona se holimo, se theha maemo a lekaneng bakeng sa ho hlahloba li-antibodies tse khotsofatsang bareki. Chate ea phallo ea tšebeletso ea ho lokisa li-antibody ea Alpha Lifetech e thehiloeng theknolojing ea ponts'o ea phage e bontšitsoe ho Setšoantšo sa 1.

Setšoantšo sa 1. Setšoantšo sa ts'ebetso bakeng sa ho lokisa li-antibodies tsa monoclonal tse thehiloeng theknolojing ea ponts'o ea phage.

Phase Display Antibody Production Workflow

Mehato	Dikahare tsa Tšebeletso	Lenane la nako
Mohato oa 1: Ho entoa liphoofolo	(1) Ho entoa liphoofolo ka makhetlo a 4, ho entoa ka matla tekanyo e le 'ngoe, kakaretso ea litekanyetso tse 5 tse entoeng. (2) Serum e mpe pele ho entoa e ile ea bokelloa, 'me ELISA e ile ea etsoa ka tekanyo ea bone ho fumana titer ea serum. (3) Haeba tekanyo ea serum antibody ea tekanyo ea bone e fihlela litlhoko, tekanyo e 'ngoe e eketsehileng ea ente e tla fanoa matsatsi a 7 pele mali a nkuoa. Haeba e sa fihlelle litlhoko, ente ea kamehla e tla tsoela pele. (4) Matla a tšoanelehang, pokello ea mali le karohano ea li-monocyte	Libeke tse 10
Mohato oa 2: Tokisetso ea cDNA	(1) PBMC Kakaretso ea ho Ntša RNA (Sete ea ho Ntša RNA) (2) Tokisetso e phahameng ea RT-PCR ea cDNA (sete ea ho ngola ka morao)	Letsatsi le le leng
Mohato oa 3: Kaho ea Laeborari ea Antibody	(1) Ho sebelisoa cDNA e le mohlala, liphatsa tsa lefutso li ile tsa eketsoa ka mekhahlelo e 'meli ea PCR. (2) Kaho le phetoho ea phage: vector ea phagemid e kopanyang liphatsa tsa lefutso, phetoho ea electroporation ea libaktheria tsa moamoheli oa TG1, kaho ea laeborari ea li-antibody. (3) Tlhahlobo: Khetha ka mokhoa o sa reroang li-clone tse 24, sekhahla sa tlhahlobo ea PCR + sekhahla sa ho kenya. (4) Ho lokisetsoa phage ka thuso: Ho eketsa phage ya M13 + ho hlwekisa. (5) Pholoso ea laeborari ea ponts'o ea Phage	Libeke tse 3-4
Mohato oa 4: Tlhahlobo ea Laeborari ea Antibody (likarolo tse 3)	(1) Tlhahlobo ea kamehla ea potoloho tse 3 (tlhahlobo ea mekhahlelo e tiileng): tlhahlobo ea khatello ho tlosa li-antibodies tse seng tse tobileng ka hohle kamoo ho ka khonehang. (2) Bacteriophage e kgethilweng ka ho eketsa klone e le nngwe+polelo e susumeditsweng ke IPTG+ho lemoha ELISA ya klone tse ntle. (3) Di-clone tsohle tse ntle di kgethilwe bakeng sa tatellano ya dijini.	Libeke tse 4-5

Melemo ea Theknoloji ea Pontšo ea phage

Alpha Lifetech e na le boiphihlelo bo bongata lefapheng la ntshetsopele ya di-antibody. Ho theosa le dilemo, Alpha Lifetech e thehile sethala se felletseng sa ntshetsopele ya di-antibody.

Litšebeletso tsa Tšehetso

Re ka fana ka lits'ebeletso tse fapaneng tsa kaho ea laeborari ea 'mele ea ho itšireletsa mafung e thehiloeng liphoofolong le lits'ebeletso tsa tlhahlobo ea laeborari ea li-antibody tsa tlhaho ho latela litlhoko tsa bareki.

Sepheo se Sengata

Litšebeletso tse ngata tsa ho sibolla li-antibody tse reretsoeng lia fumaneha: liprotheine, li-peptide, limolek'hule tse nyane, livaerase, liprotheine tsa lera, mRNA, jj.

Li-vector tse ngata

Tšebeletso ea kaho ea laeborari e ikhethileng, re ka fana ka li-vector tse fapaneng tsa bacteriophage ho kenyeletsoa PMECS, pComb3X, le pCANTAB 5E, 'me ra li fetola ho latela litlhoko tsa bareki.

Sethala sa Batho ba Holileng

Bokgoni ba polokelo bo ka fihla ho 10 ^ 8-10 ^ 9, sekgahla sa ho kenngwa kaofela se ka hodimo ho 90%, mme kamano ya di-antibodies tse fumanwang ka ho hlahlojwa hangata e maemong a nM pM.

TŠEBELETSO E Amanang

Maano a mangata a nts'etsopele ea li-antibody

Tšebeletso ea Ntlafatso ea Li-antibody tsa Monoclonal

Sethala sa Theknoloji ea Hybridoma

Sethala sa ho Hlopha Lisele tse le 'Ngoe tsa B

Sethala sa Ntlafatso ea Antibody sa Pontšo ea Phage

Nts'etsopele ea antibody ea monoclonal - Alpha Lifetech

Tšebeletso ea Ntlafatso ea Li-antibody tsa Monoclonal

Re ka fana ka lits'ebeletso tsa nts'etsopele ea li-antibody tsa monoclonal tsa boleng bo holimo, tse hloekileng haholo le tse ikhethileng haholo, ho kenyeletsoa tlhahiso ea li-antibody tsa monoclonal tsa toeba le li-antibody tsa monoclonal tsa mmutla.

Sethala sa Theknoloji ea Hybridoma

Ho kenyeletsoa lenaneo la ho enta, lits'ebeletso tsa ho lokisa li-antibody, tlhoekiso ea li-antibody, tatellano e phahameng ea li-antibody, netefatso ea li-antibody, jj.

tlhahlobo ea lisele tsa b-Alpha Lifetech

Sethala sa ho Hlopha Lisele tse le 'Ngoe tsa B

Alpha Lifetech e na le melemo ea nako ea tlhahlobo le ho fumana li-antibodies tsa boleng bo holimo. E ka fana ka moralo oa antigen, tlhahiso le phetoho, tšireletso ea liphoofolo, tlhahlobo ea ntlafatso ea sele e le 'ngoe ea B, tatellano ea sele e le 'ngoe.