Selelekela ho Lilaebrari tsa Synthesized Antibody

Laeborari ea synthesized antibody, eo hape e tsejoang e le laeborari ea De novo, ke mokhoa oa maiketsetso o sebelisang mekhoa e kang DNA synthesis kapa phage display ho rala le ho kopanya libaka tse fapaneng tsa antibody, ho kenyeletsoa libaka tsa moralo le li-CDR, ntle le ho itšetleha ka lilaeborari tsa naïve antibody.

Laeborari ea li-Semi-synthetic antibody e entsoe ka ho kopanya lilaebrari tsa li-antibody tse hlahang ka tlhaho le mefuta-futa ea maiketsetso. Hangata e kenyelletsa ho kopanya li-oligonucleotide tsa DNA ho hlahisa libaka tse fapaneng tse tlatselletsang tse khethollang (CDRs), tseo ka nako eo li kopantsoeng le moralo oa kamehla oa antibody o tsoang mehloling ea tlhaho joalo ka lisele tsa B tsa motho kapa tsa liphoofolo. CDR e kopaneng e hlahisa mefuta e fapaneng ka har'a laeborari, e bakang li-antibodies tse shebileng mefuta e mengata ea li-epitopes. Laeborari ea li-anti-synthetic antibody e fana ka sekisetsa lipakeng tsa mefuta-futa ea tlhaho ea sesole sa 'mele le phapano e laoloang e fihlelletsoeng ka mekhoa ea maiketsetso.

Phapang pakeng tsa lilaebrari tsa naïve le tsa maiketsetso tsa antibody e itšetlehile ka mohloli oa liphatsa tsa lefutso tsa immunoglobulin. Ho sa le joalo, phapang lipakeng tsa laeborari ea li-anti-synthesized semi-synthesized le synthesized antibody e teng ka hore ea pele e na le karolo ea likarolo tsa naïve antibody, joalo ka ketane e bobebe kapa ketane e boima, mme e le 'ngoe feela ea tsona e entsoe ka in vitro; ha e entsoeng ka maiketsetso e nkiloe ka botlalo ho tsoa ho maiketsetso a PCR in vitro.

Alpha Lifetech e ka fana ka

Litlhaloso tsa likarolo tsa Alpha Lifetech Inc.e ka fana ka laeborari ea li-anti-synthesized semi-synthesized/ synthesized antibody e tsoang liphoofolong le bathong e ipapisitseng le sethala sa rona sa profeshenale sa ho sibolla antibody. Ka boiphihlelo ba lilemo tsa phetoho ea liphatsa tsa lefutso le kaho ea laeborari, lilaebrari tse kopaneng li ka hahoa tse khonang ho hlahisa li-antibodies tsa maiketsetso tse nang le kamano le tse ikhethang ho feta matla a li-antibodies tsa tlhaho.Alpha Lifetech'slitsebi li motlotlo ho phatlalatsa hore re ka netefatsa katleho e phahameng kahong ea laebrari ea li-antibody e nang le li-clone tse ikemetseng tsa 10^8 - 10^10.

Litlhaloso tsa likarolo tsa Alpha Lifetech Inc.e motlotlo ho fana ka lits'ebeletso tse felletseng tsa kaho ea laeborari ea antibody ho fihlela litlhoko tse fapaneng tsa bafuputsi ba lefats'e, ho kenyeletsoa scFv, Fab, VHH antibody, le lilaeborari tse ikhethileng. Sepheo sa rona ke ho utloisisa le ho fihlela litlhoko tsa bareki ba fapaneng le ho thusa ka mathata afe kapa afe a tlang le a hlahang mosebetsing oa lipatlisiso.

Ts'ebetso ea Ts'ebeletso ea Kaho ea Lilaebrari tsa Antibody tse Kopantsoeng

Moralo
Mokhahlelo oa moralo o kenyelletsa ho khetha li-antibody frameworks le complementarity-determining regions (CDRs) ho ipapisitsoe le tatellano e teng ea li-antibody kapa data ea sebopeho. Lisireletsi tsa maiketsetso tsa CDR le tsona li ka etsetsoa ho hlahisa tšebetso e itseng kapa ho ntlafatsa thepa e tlamang.

Synthesis
Synthetic DNA e khoutong tatellano ea li-antibody e etselitsoeng, ho kenyeletsoa libaka tse peli tsa moralo le li-CDR, e entsoe ka mekhoa ea lik'hemik'hale kapa ea enzymatic.

Kopano
Likhechana tsa DNA tse kopaneng li bokelloa ka har'a li-antibody expression vectors ho sebelisoa mekhoa e kang PCR, ligation, kapa Gibson assembly. Li-vector tsena li ka kenngoa lits'ebetsong tsa polelo tse kang baktheria, yeast, kapa lisele tsa mammalian bakeng sa tlhahiso ea antibody.

Ho lekola le ho khetha
Lilaebrari tse entsoeng tsa li-antibody lia hlahlojoa le ho khethoa bakeng sa li-antibodies tse nang le thepa e lakatsehang ka mekhoa e phahameng ea ho hlahloba. Sena se ka kenyelletsa mekhoa e kang pontšo ea phage, pontšo ea tomoso, kapa pontšo ea ribosome, ho itšetlehile ka sebopeho sa laebrari le ts'ebeliso.

Ts'ebeletso ea Kaho ea Lilaebrari tse Kopantsoeng tsa Antibody

Litlhaloso tsa likarolo tsa Alpha Lifetech Inc.e fana ka bareki ka moralo o le mong le ts'ebeletso e kopaneng ea lilaebrari tsa antibody ka ho kopanya li-repertoires tsa V-gene tse boima le tse bobebe ho vector ea phage ho sebelisa sistimi e ikhethileng ea sebaka sa Cre-lox ho theha pokello ea masela a bonts'itsoeng phage e nang le 6.5 × 10 ^ 10 clones. Laeborari e ile ea hlahisa Abs khahlanong le li-antigen tse ngata, tse ling tse nang le li-nanomolar affinities. Moloko oa laebrari ea antibody ea motho e thehiloe ho thekenoloji ea pontšo ea M13 phage (e bontšitsoeng setšoantšong se ka tlase).