Intshayelelo yeNkonzo yoBoniso lweZigaba

Ukuboniswa kwePhage, njengendlela yemolekyuli esekelwe kutshintsho lwemfuza lwe-phage DNA, kuye kwaba sisixhobo esinamandla semolekyuli ukuvelisa iiprobe zemolekyuli ngokuchasene neethagethi ezithile ngokubonisa ezi ziqwenga kumphezulu wephage kwaye ngaloo ndlela ukhethe iziqwenga zepeptide/antibody ezineempawu ezithile zokubopha kwiindidi ezininzi (iilayibrari).

I-Alpha Lifetech inamava eminyaka elishumi ekuphuhliseni ii-antibodies ze-phage display kwaye inokubonelela ngenkonzo yobuchwephesha yokwakha kunye nokuhlola ithala leencwadi le-antibody kubathengi bethu. Ngokweemfuno zabathengi, singabonelela ngeendlela ezahlukeneyo zeelayibrari ze-antibody, ezifana neelayibrari ze-immune, iilayibrari zemveli, iilayibrari zokwenziwa kunye neelayibrari ze-semi-synthetic. Itekhnoloji yelayibrari ye-Phage display, edibanisa ii-genes ze-antibody kunye nee-genes zeeproteni ze-phage coat ezifana ne-M13, yeyona teknoloji ixhaphakileyo yokulungiselela ii-antibody kwaye isetyenziswa kakhulu ekuphuhliseni ii-antibodies ezithile.

I-Alpha Lifetech iza kugonya i-alpaca, i-llama, i-alpaca, kunye neeshark, njl.njl., ukuze ukusebenza kwazo kufikelele kwi-10^5. Siza kuthumela ingxelo yovavanyo lokusebenza kumthengi ukuqinisekisa ukuba idatha iyasebenza kwaye iyinyani. Iilayibrari zemveli zineengenelo zokuba netyhefu ephantsi kunye nokungasebenzi kakuhle kwe-immunogenicity. I-Alpha Lifetech inenani elikhulu leelayibrari ze-antibody ezenziwe kwangaphambili, ezinokusetyenziselwa ngokuthe ngqo ukuvavanya i-antibody ngaphandle kokugonya izilwanyana, ngaloo ndlela zinciphisa ixesha leprojekthi, ziqhubele phambili inkqubela phambili yovavanyo lomthengi, kwaye ziqinisekisa ukusebenza kakuhle kwe-antibody.

ukufunyanwa kwee-antibody-Alpha Lifetech

Umfanekiso 1: Umgaqo wokubonisa iiPhage

Inkqubo yethu yoBoniso lweePhage

Ulwazi oluphambili lwenkqubo yokubonisa i-M13/T4/T7 phage luboniswe kwiTheyibhile 1.

Itheyibhile 1 Izithuthi ezahlukeneyo zokubonisa iiphage kunye neempawu zazo
	M13	T4	T7
Ubungakanani beJini	6407 bp	168895 bp	39937 bp
Bonisa iiProteni	i-pVI, i-pIII kunye ne-pVIII	I-SOC kunye ne-HOC	gp10B
Ubungakanani boBoniso	>110 kDa yi-pIII
Ubungakanani boBoniso
Ubuninzi beDisplay		Iikopi ezingama-810 kwi-SOC	Iikopi ezingama-415
Ubuninzi beDisplay	Iikopi ezingama-2700 kwi-pVIII	Iikopi ezingaphantsi kwe-155 kwi-HOC	Iikopi ezingama-415
Umnqa wobomi	I-Lysogeny	ILytic	ILytic

I-Alpha Lfetech Ingabonelela

I-Phage Display idibanisa i-genotype kunye ne-phenotype zibe yinto enye, idibanisa ukukhetha kunye nokukhulisa kunye nobuchule obunamandla bokuhlola. I-Alpha Lifetech inokubonelela ngeenkonzo ezahlukeneyo zophuhliso lwe-antibody ye-phage display.
Iinkonzo eziphambili ziquka: iqonga lelayibrari ye-antibody ye-VHH, iqonga lelayibrari ye-antibody ye-scFv, iqonga lelayibrari ye-antibody ye-Fab, iqonga lokwakhiwa kwelayibrari ye-phage, iqonga lokuhlola ilayibrari ye-phage kunye nenkonzo yokwenza i-antibody ibe ngumntu kunye nezinye iinkonzo.

Umfanekiso 2: Inkqubo yenkonzo yophuhliso lomboniso wePhase

Inkqubo yoPhuhliso lwe-Antibody ye-Phage Display

Singagonya izilwanyana ngeeproteni ezivezwa kwilebhu yethu okanye ezibonelelwa ngabathengi bethu, kuquka iiproteni ezahlukeneyo ze-macromolecular, iipeptides kunye neeproteni ze-membrane. Ngovavanyo lokusebenza lwe-ELISA, abaphandi bethu benze i-PBMC yokwahlulwa kunye nokukhutshwa kwe-RNA egazini le-alpacas ngeziphumo ezilungileyo zokugonywa. Ngokuguqulelwa okungasemva kunye nokwandiswa kwe-gene, sakhe i-gene kwi-pMESC, i-pComb3XSS okanye i-pCANTAB 5E phage vectors. Ngokuguqulwa kwe-electrotransformation yeeseli ezikwaziyo ze-TG1 E. coli, iilabhulali ze-phage antibody ezinomthamo wethala leencwadi ongaphezulu kwe-10^9 zinokufunyanwa. Iilabhulali ze-antibody ezinomthamo omkhulu wethala leencwadi zinokwahluka okuhle kwaye zinceda ukufumana ii-antibodies ezine-affinity. Ukuqinisekisa umgangatho wethala leencwadi le-antibody, siza kuchonga umthamo wethala leencwadi kunye nesantya sokufakwa kwethala leencwadi. Ekugqibeleni silandelelanisa iilabhulali ukuze sinike abathengi bethu ulandelelwano olufunekayo lwe-30-50. Emva koko, senza uvavanyo lwelayibrari yokubonisa i-phage, Okokuqala, i-antigen ayishukumi kwii-microplates ze-polypropylene, kwaye ngemijikelo emi-3-5 yovavanyo, ii-phage-antibodies ezinokukwazi ukubopha okubuthathaka ziyasuswa, kwaye ii-clones ezithile ezibopha kwi-antigen ziyagcinwa. Kwi-phage display, indlela ye-ELISA isetyenziselwa ukugqibela nge-clone elungileyo, ngelixa kwi-yeast display, indlela ye-FACS isetyenziselwa ukwenza iiseli zibonise iziqwenga ze-antibody eziphinda-phindayo sisebenzisa ii-antigens ezibhalwe amagama. Ngokuhlola, silungiselela ii-antibodies ezinovakalelo oluphezulu, ukucacisa kunye nobudlelwane obuphezulu.

Umfanekiso 3 Inkqubo yokwenziwa kwe-antibody yokubonisa i-Phage display

IINKONZO ZENKONZO
Iingenelo zeNkonzo

I-Alpha Lifetech izimisele kakhulu kubuchwepheshe bokubonisa i-phage, kwaye inokubonelela abathengi ngeenkonzo ezibanzi nezingcono ze-phage.

Ixesha kunye nokusebenza kakuhle okuphezulu

Inkampani yethu inokukhangela ii-antibodies ezanelisayo ngexesha elifutshane; singenza iimvavanyo ezahlukeneyo zokuhlola ii-antibody ngokweemfuno zabathengi.

Umgangatho ophezulu wemveliso

Inkampani yethu inceda abathengi ukulungiselela ii-antibodies ezineenkcukacha eziphezulu kunye nozinzo, nto leyo enyusa iziphumo zophando lwesayensi.

Nika ukhetho olwahlukeneyo

Inkampani yethu inokwakha iilabhulali zee-antibody zeentlobo ngeentlobo (zomntu, impuku, umvundla, i-alpaca, njl.njl.) kwaye inokwakha iintlobo ngeentlobo zeelabhulali zee-antibody (scfv, Fab, VHH, njl.njl.).

Umthamo Omkhulu

Ithala lethu leencwadi zee-antibody linomthamo omkhulu wee-antibodies ezingaphezu kwe-10^9

Imibuzo ebuzwa rhoqo-Iingxaki ezibanzi zokubonisa i-phage

Imibuzo yokubonisa i-phage ◢

Umbuzo.
Yintoni iteknoloji yokubonisa i-phage?

A.
Itekhnoloji yokubonisa iPhage kukufaka i-gene yangaphandle kwindawo ethile yeprotheyini ye-phage capsid, kwaye iveze i-gene yangaphandle kumphezulu we-phage ngaphandle kokuchaphazela umsebenzi oqhelekileyo we-phage. Emva kwezihlandlo ezininzi zokuhlola, iprotheyini ekujoliswe kuyo ene-specific enamandla kunye nomsebenzi ophezulu webhayoloji ekugqibeleni yafunyanwa.
Umbuzo.
Ziziphi iingenelo zobuchwepheshe bokubonisa i-phage?

A.
Iilayibrari ze-antibody ezinomthamo ophezulu zinokwenziwa kusetyenziswa obu buchwephesha. Umjikelo wokuhlola umfutshane, inkqubo yokusebenza ilula, kwaye uluhlu lwezicelo lubanzi, okuvumela ukuchongwa kolandelelwano olufanelekileyo lweepeptides okanye iiproteni ezijoliswe kuzo. Kwizicelo ezinxulumene neentsholongwane, abaphandi banokufumanisa ii-antibodies ezibopha kwii-epitopes ezibalulekileyo zentsholongwane ngokuhlola iilayibrari ezinkulu ze-antibody. Le nkqubo inokuthintela ukungena, ukuphindaphinda, okanye iindlela zokukhusela amajoni omzimba kwiintsholongwane, ngaloo ndlela iseka isiseko sonyango lwesifo.
Umbuzo.
Yintoni umahluko phakathi kobuchwepheshe be-hybridoma kunye nobuchwepheshe be-phage?

A.
Iteknoloji yokubonisa i-phage isetyenziswa kakhulu kuneteknoloji ye-hybridoma, ekhawulelwe ekuveliseni inani elincinci lee-antibodies ezichasene ne-immunogens ezithile. Ngokwahlukileyo koko, iteknoloji yokubonisa i-phage inokubonisa ithala leencwadi elipheleleyo lee-antibody ezivela kwiintlobo ezahlukeneyo ze-immune.
Umbuzo.
Zithini iindlela zokusebenzisa ubuchwepheshe bokubonisa i-phage?

A.
Itekhnoloji yokubonisa iiphage ineendlela ezahlukeneyo zokusebenza, kuquka ukuhlola ii-receptors zeentsholongwane ezibangela izifo, ukufunda ukusebenzisana kweeproteni, ukulandelela ukudluliselwa kwesignali phakathi kweeseli, ukufumanisa izifo zezilwanyana, kunye nokuphuhlisa amayeza okugonya, phakathi kwezinye iinkalo.
Umbuzo.
Ithemba lobuchwepheshe bokubonisa i-phage?

A.
Itekhnoloji yokubonisa iiphage inika iingenelo ezininzi kwaye ingasetyenziswa kwiinkalo ezahlukeneyo. Nangona kunjalo, umceli mngeni ophambili ekuphuhliseni ii-antibodies eziphinda-phindayo kusetyenziswa indlela yokubonisa iiphage bubunzima kunye neendleko ezinxulumene nale nkqubo. Le ndlela ifuna ukuhlanganiswa kweendlela zebhayoloji yeemolekyuli, kubandakanya ukuguqulwa kwezakhi zofuzo, ukwenziwa kwe-cloning, kunye nokubonakaliswa, kunye nohlalutyo lwe-immunological, biochemical, kunye ne-biophysical ukuchonga kunye nokuchonga ii-antibodies ezifunekayo.

Imibuzo enxulumene ne-Antigen ◢

Umbuzo.
Ziziphi iindlela zokubonisa i-antigen?

A.
Ukubonakaliswa kwe-antigen lukhonkco olubaluleke kakhulu. Iindlela zokubonakaliswa kwe-antigen ziquka ikakhulu ukubonakaliswa kwe-antigen egqunywe ngqo, ukubonakaliswa kwe-antigen egqunywe ngokungangqalanga, ukubonakaliswa kwe-antigen ngokuhlolwa kweseli yonke, ukubonakaliswa kwe-antigen nge-liposomes, ii-nano-phospholipid disks kunye nee-VLPs.
Umbuzo.
Zithini umahluko phakathi kweentlobo ezahlukeneyo zokubonakaliswa kwe-antigen?

A.
Indlela yokugquma ngokuthe ngqo ilula kakhulu, kodwa ukwakheka kwe-antigen kulula ukuyitshintsha, kwaye ukusebenza kokugquma okungathanga ngqo kuyinkimbinkimbi, kodwa kunokugcina ukwakheka kwe-antigen kwaye kuphucule ukusebenza kakuhle kokubonakaliswa. Ukuhlolwa kweeseli kunokulinganisa imeko yokwenyani kwi-vivo, kodwa kukho ingxaki yokubopha okungangqalanga. Idiski ye-Nano-phospholipid inobukhulu obuncinci, ukusebenza kakuhle kokuhanjiswa kunye nozinzo oluhle; ii-VLP zixutywe ne-antigen okanye zisongelwe nge-antigen, enomzimba ophezulu kunye nokhuseleko oluhle, kwaye inokubangela impendulo enamandla yomzimba.
Umbuzo.
Iyintoni impembelelo yoxinzelelo lwe-antigen kwiziphumo zovavanyo lwetekhnoloji yokubonisa i-phage?

A.
Ukuba uxinzelelo luphantsi kakhulu, amathuba okubopha kunye ne-phage aya kuncipha, nto leyo ekhokelela ekunciphiseni ukusebenza kakuhle kokuhlola. Ngokwahlukileyo koko, uxinzelelo oluphezulu kakhulu lunokunyusa amathuba okubopha okungaqhelekanga, lunyuse isignali yangasemva, kwaye luthintele ukuchongwa kwee-clones ezilungileyo.
Umbuzo.
Singaqinisekisa njani ukuba i-antigen igcina ukwakheka kwayo kwendalo ngexesha lenkqubo yokuvavanya?

A.
Indlela yokulungisa ethambileyo ingasetyenziswa ukuze kuthintelwe ubushushu obuphezulu, i-asidi enamandla kunye ne-alkali. Indlela yokwambathisa engathanga ngqo kunye nenkqubo ye-biotin-streptavidin zisetyenzisiwe ukunciphisa umonakalo kwisakhiwo se-antigen. Ukuba i-antigen yiproteni ye-membrane, ingaboniswa kumphezulu weeseli ezingasebenziyo okanye isetyenziswe ukulinganisa imeko-bume ye-membrane, njenge-nano-disk, ukugcina ukwakheka kwayo kwendalo kweproteni ye-membrane.
Umbuzo.
Ungaqinisekisa njani ukuchaneka kokubopha kwee-antibodies ezihlolwe yiteknoloji yokubonisa i-phage kwi-antigens?

A.
Ukongeza kwiimvavanyo eziqhelekileyo ezifana ne-ELISA, i-Western Blotting (WB), kunye ne-Immunofluorescence (IF), iindlela zokufumanisa i-Surface Plasmon Resonance (SPR) kunye ne-Bio-Layer Interferometry (BLI) zingasetyenziswa ukujonga iinkqubo zokubopha kunye nokwahlukana kwee-antibodies kunye nee-antigens ngexesha langempela, okuvumela ukuqinisekiswa kwe-affinity kunye ne-kinetic parameters.

Imibuzo Enxulumene Nomzimba Ongasebenziyo ◢

Umbuzo.
Zithini iimpendulo zomzimba ngokubanzi zezilwanyana kubuchwepheshe bokubonisa i-phage?

A.
Ukuboniswa kwe-Phage kungasetyenziselwa ukuvelisa ii-antibodies ze-monoclonal ezihambelana kakhulu kwiintlobo ngeentlobo zeentlobo ezivelisa ii-antibodies, kuquka kodwa kungaphelelanga apho kubantu, iimpuku, iimpuku, imivundla, iinkukhu, iinkamela, ii-alpaca, iinkomo, izinja, iigusha, iinkawu, kunye nooshaka.
Umbuzo.
Ziziphi iimpawu kunye neemeko ezifanelekileyo zeelayibrari ze-antibody ezithathwe kwiintlobo ngeentlobo zezilwanyana kwitekhnoloji yokubonisa i-phage?

A.
Itekhnoloji yokwakha iilayibrari ze-antibody yegundane imiselwe kakuhle, ingabizi kakhulu, kwaye inobude obufutshane bovavanyo, nto leyo eyenza ukuba ifaneleke ukuhlolwa kwe-antibody yokuqala kunye nophando olusisiseko. Ii-antibodies zemvundla zibonisa ulwalamano oluqinileyo kunye nokucaciswa, kunye noluhlu olubanzi lokuqatshelwa kwe-epitope xa kuthelekiswa nee-antibodies zegundane. Ii-antibodies zabantu zinokufunyanwa ngokuthe ngqo kwiilayibrari ze-antibody zabantu, ngaloo ndlela kuthintelwe iimpendulo ezinokubakho zokuzikhusela komzimba ezinokwenzeka xa ii-antibodies ezahlukeneyo zinikwa umzimba womntu. Le ndlela ixabisa kakhulu kuphando nophuhliso lwamayeza, ngakumbi ekudalweni kwee-antibodies zonyango.
Umbuzo.
Yintoni i-immunogen yezilwanyana ngokubanzi esetyenziswa kwiteknoloji yokubonisa i-phage?

A.
Ii-immunogens zezilwanyana ngokubanzi zahlulwe zibe yi-protein antigen, i-polypeptide antigen, i-nucleic acid antigen kunye ne-pathogen-related antigen, phakathi kwazo i-pathogen-related antigen iquka i-virus antigen (i-complete virus particle, i-virus protein, i-virus non-structural protein, njl.njl.), i-bacterial antigen (complete bacterial extract, cell wall component, i-secreted protein, i-toxin, njl.njl.) kunye ne-parasite antigen.
Umbuzo.
Singawujongana njani nomba wokumelana nomzimba kwizilwanyana ngexesha lokusabela komzimba?

A.
Lungisa ii-immunogens ngokufaka ii-antigens zokuzikhusela komzimba ngeeproteni eziphinda zihlanganiswe emzimbeni kwaye ufake iiproteni zesakhiwo seentsholongwane endaweni yee-antigens ze-antigens ezilwa nomlilo. Lungisa uyilo lovavanyo kwaye utshintshe umthamo wokuzikhusela komzimba.
Umbuzo.
Ingaphuculwa njani indlela ii-immunogenicity ze-immunogens ezisebenza ngayo kubuchwepheshe bokubonisa ii-phage?

A.
Ukuqina kwe-immunogenicity kunokuphuculwa ngokudibanisa ii-antigens kunye neeproteni ezithwala, ezifana ne-bovine serum albumin. Ukongeza, izixhobo zokuncedisa i-immune, kuquka i-Freund's adjuvant kunye ne-aluminium adjuvant, zingasetyenziswa ukwandisa ixesha lokuhlala emzimbeni kwaye zikhuthaze ukusebenza kunye nokwanda kweeseli zomzimba, ngaloo ndlela ziphucula ukuqina kwe-immunogenicity.

Imibuzo Yokwakha Ithala Leencwadi ◢

Umbuzo.
Yintoni ithala leencwadi le-phage antibody?

A.
Ithala leencwadi le-phage antibody yingqokelela yee-antibodies ezahlukeneyo ze-phage ezenziwe ngokudibanisa iseti epheleleyo yee-genes zengingqi eziguquguqukayo ezivela kwii-antibodies zibe zii-phage vectors kwaye zizivezwe kumphezulu wee-phages. Le nkqubo iphumela ekwakhiweni kwethala leencwadi le-phage antibody.
Umbuzo.
Inkqubo yokwakha ithala leencwadi le-phage antibody

A.
Inkqubo eqhelekileyo yokuboniswa kwe-phage ibandakanya amanyathelo aliqela abalulekileyo: ukukhupha iiseli ze-peripheral blood mononuclear (PBMC) kwizilwanyana ezigonyiweyo, ukwahlula i-RNA iyonke nokuyiguqulela kwi-DNA eyongezelelekileyo (cDNA), ukwandisa i-target gene kusetyenziswa ubuchwepheshe be-polymerase chain reaction (PCR), ukuhlanganisa i-target gene ibe yi-phage vector, kunye nokuguqula i-vector ibe yiseli yomninimzi ukuze ibonakale. Le nkqubo iphumela ekwakhiweni kwethala leencwadi lokuboniswa kwe-phage, elinokuchonga ngokukhawuleza ii-antibodies ezihambelanayo. Emva koko, eli thala leencwadi linokusebenza njengomthombo obalulekileyo wophuhliso lwezitofu, amayeza onyango, kunye nee-reagents zokuxilonga.
Umbuzo.
Ziziphi iingongoma eziphambili zokwakha ilayibrari esemgangathweni ophezulu?

A.

Qinisekisa ukuba inani lilonke le-PBMC lanele. Qinisekisa umgangatho we-RNA ngaphandle kokuwohloka.

Ukuqinisekisa inani lilonke lee-transformants, umthamo wethala leencwadi ngokubanzi kufuneka ube ngokuphindwe kalishumi ukuya kwi-100 kunokwahluka kwethiyori.
Umbuzo.
Ziziphi iindidi zethala leencwadi le-phage antibody?

A.
Ngokutsho komthombo welayibrari ye-antibody, iilayibrari ze-phage antibody zinokuhlulwahlulwa zibe ziilayibrari ze-immune phage antibody kunye neelayibrari ze-native phage antibody. Umahluko ophambili phakathi kwezi ntlobo zimbini kukuba izilwanyana zigonyiwe na. Ukongeza, iilayibrari ze-native antibody zinokwenziwa ngokusebenzisa i-semi-synthesis okanye i-synthesis.
Umbuzo.
Umntu angawuvavanya njani umgangatho wethala leencwadi le-phage antibody?

A.
Kukho izalathisi ezimbini eziphambili zokuvavanya umgangatho wethala leencwadi le-antibody: umthamo wokugcina kunye nokwahluka. Umthamo omkhulu wokugcina, kunye nokwahluka okuphezulu, kuqinisekisa ukuhlolwa okusebenzayo kwee-antibodies zombini ukuhambelana okuphezulu kunye nokucaciswa.

Imibuzo yoHlolo lweThala leeNcwadi ◢

Umbuzo.
Iyintoni inkqubo yokuvavanya ithala leencwadi le-phage antibody?

A.
Kwinkqubo yokuhlaziya izinto eziphilayo, ithala leencwadi elibonisa izinto lifakwa kwiithagethi ezizinzileyo lize emva koko lihlanjwe kakhulu ukuze kususwe ii-phages ezingasebenziyo. Ii-Conjugates zihlala zisuswa kusetyenziswa isisombululo esine-asidi okanye ityuwa eninzi kwaye emva koko zikhuliswe kwiiseli ezifanelekileyo zommkeli ukuze zityebiswe. Emva kwemijikelo emi-3 ukuya kwemi-5 yokuhlolwa, ii-antibodies ezine-affinity ephezulu ziyafunyanwa.
Umbuzo.
Ziziphi iindlela ezisetyenziswayo ekuhloleni iilayibrari ze-phage antibody?

A.
Iindlela zokuhlola iiphage ziquka ikakhulu i-solid-phase panning, i-liquid-phase screening, kunye ne-cell-based screening. Ukukhethwa kwendlela efanelekileyo yokuhlola kufuneka kuqwalasele iimpawu zeemolekyuli ekujoliswe kuzo, iinjongo zenkqubo yokuhlola, kunye nemeko-bume yelabhoratri.
Umbuzo.
Zithini iziphumo ezilungileyo nezingalunganga zokuhlolwa kwee-phages?

A.
Ukuhlolwa okuqinisekileyo yeyona ndlela iphambili yokuvavanya ii-antibodies ze-phage ezibopha kwiiproteni ezijoliswe kuzo. Ukuhlolwa okungalunganga kusebenza ekususeni ii-antibodies ezingezizo ezithile. Ngexesha lenkqubo yokuhlolwa kwee-antibody ze-phage, ii-antibodies ezingezizo ezithile zinokuchongwa ngenxa yokuphazamiseka okuvela kwileyibhile ye-antigen ekujoliswe kuyo, izinto, okanye ezinye ii-antigens ezifana nolwakhiwo lwe-antigen ekujoliswe kuyo. Kwiimeko ezinjalo, ukuhlolwa okungalunganga kunokuphucula ukusebenza kakuhle kwenkqubo yokuvavanya.
Umbuzo.
Ungaziphepha njani iimvavanyo zee-antibodies ezingezizo ezithile?

A.

Ii-antigens ezibhalwe ngeentlobo ezahlukeneyo zeemaki zinokubekwa phambili xa kusenziwa uvavanyo lokuqala, okanye kusetyenziswe uvavanyo olubonisa ukuba azikho iintsholongwane ezithile.

Ungakhetha ukutshintsha ulwelo lokutywina olufanelekileyo okanye usebenzise ulwelo lokutywina olwahlukileyo ukuthintela le meko. Ukuba i-antibody eqaphela umgca weseli yangasemva (i-CHO okanye i-HEK293) ihlolwe, ungathatha indawo yemigca yeeseli yangasemva eyahlukileyo ngenjongo yokuvavanya okanye yokuvavanya okungalunganga.
Umbuzo.
Umntu angaqinisekisa aze ahlalutye njani ii-antibodies ezifunyenweyo?

A.
Iindlela zokuqinisekisa i-antibody zihlala ziquka i-ELISA, i-Western Blotting (WB), i-flow cytometry, i-immunoprecipitation (IP), i-immunofluorescence (IF), i-immunohistochemistry, kunye nezinye iindlela ezahlukeneyo zovavanyo. I-Alpha Lifetech inamava amaninzi ekufumaneni i-antibody kwaye ineqela leengcali zovavanyo, ukuqinisekisa ukusebenza kakuhle kwe-antibodies kwizilingo ezilandelayo.

Ukuba unemibuzo, nceda ukhululeke ukunxibelelana nathi nangaliphi na ixesha.

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Inkonzo Ebalaseleyo

Iqonga lokuFumanisa iiAntibody