Inkonzo yoHlalutyo lweempawu ze-Aptamer

I-Alpha Lifetech ibisoloko inika iinkonzo ezinxulumene ne-aptamer kangangeminyaka emininzi, kwaye okwangoku inokubonelela ngezicwangciso zophuhliso ezibanzi ze-aptamer ezifana nokuhlanganiswa kwe-aptamer, ukuhlolwa kwe-SELEX, ukulandelelana okuphezulu, kunye nokwenza ngcono kunye nokuchazwa kwe-aptamer. Ngokusekelwe kwiingenelo zendlela ye-SELEX yokuhlola ii-aptamers, i-Alpha Lifetech yandise iindlela ezininzi zokuhlola ii-aptamers.

Intshayelelo kuHlalutyo lweempawu ze-Aptamer

Ukuqinisekiswa kobudlelwane

I-aptamer affinity ephuculweyo iqinisekiswe ngeendlela ezifanelekileyo zovavanyo lwe-aptamer binding. Imizekelo ibandakanya i-Isothermal Titration Calorimetry (ITC), i-Flow Cytometry (FCM), kunye ne-Surface Plasmon Resonance (SPR), i-Microfluidics, njl. I-aptamer affinity idla ngokubonakaliswa yi-dissociation constant (KD), ubungakanani obuphathekayo obusetyenziselwa ukubala inqanaba le-molecular dissociation kwi-reversible reaction. Okukhona ixabiso le-KD lincinci, kokukhona i-complex izinze ngakumbi, oko kukuthi, i-affinity inamandla ngakumbi; Ngokwahlukileyo koko, okukhona ixabiso le-KD likhulu, kokukhona i-complex ingazinzi kwaye i-affinity ibuthathaka. Eli nyathelo libalulekile ekuchongeni ukusebenza kwe-aptamer, ukuqinisekisa ukuvuthwa kwe-aptamer affinity kwaye inokubopha kwi-molecule ekujoliswe kuyo nge-affinity ephezulu kunye nokukhetha kwizicelo ezisebenzayo.

Umfanekiso 1 Inkqubo yokuvuthwa kwe-aptamer affinity. Umthombo wesalathiso:IKinghorn AB, eFraser LA, ngo-2017.

Ukuqinisekiswa komsebenzi

Ukongeza ekuqinisekisweni kwe-affinity, umsebenzi we-aptamer kufuneka uqinisekiswe. Oku kuquka ukuqinisekisa uzinzo lwe-aptamer, ukuchaneka kwayo, kunye nokusebenzisana kwayo nezinye iimolekyuli kwindawo ethile. Iziphumo zokuqinisekiswa kokusebenza ziya kuchaphazela ngokuthe ngqo ixabiso le-aptamers kuphando lwesayensi kunye nokusetyenziswa kwemizi-mveliso.

Uqinisekiso oluthile

Uvavanyo lokukhuphisana: Amandla e-aptamer okubopha kwithagethi ethile avavanywa xa kukho ezinye iithagethi ezifanayo. Ukuba i-aptamer inokubopha ngokukodwa kwi-molecule ekujoliswe kuyo ngaphandle kokuphazamiseka kwezinye ii-molecule, oko kubonisa ukuba inobungcali obuphezulu.

Uvavanyo lwe-Cross reaction: I-aptamer ibotshelelwe kuluhlu lweethagethi ezinxulumeneyo okanye ezingadibaniyo ukuze ibone ukuba ibotshelelwe kuphela kwithagethi ethile, ukuze kuqinisekiswe ukuba icacile.

Ukuqinisekiswa kozinzo

Uvavanyo lokubola kwe-nuclease: Ii-aptamers zavezwa kumanqanaba ahlukeneyo e-nucleases kwaye ukubola kwazo kwabonwa. Ngokuthelekisa inqanaba lokubola ngamaxesha ahlukeneyo, amandla okulwa nokubola kwe-nuclease ye-aptamer anokuvavanywa.

Uvavanyo lokuqina kobushushu kunye nexesha: I-aptamer ibekwe phantsi kobushushu obahlukeneyo kunye neemeko zexesha ukuze ibone uzinzo lwayo lwesakhiwo kunye nokusebenza kwayo. Oku kunceda ekumiseleni iimeko ezifanelekileyo zokugcina nokusetyenziswa kwe-aptamers.

Ukuchongwa komsebenzi webhayoloji

Indlela efanelekileyo idla ngokukhethwa ngokwenjongo ethile yokusetyenziswa kwe-nucleic acid aptamer.

(1) Uvavanyo lwenqanaba leMolekyuli: Ukusebenzisa iindlela zebhayoloji yemolekyuli, ezifana ne-gel electrophoresis, i-Western blot, njl.njl., ukufumanisa ii-complexes ezenziwe emva kokuba i-aptamer idibene nemolekyuli ekujoliswe kuyo, okanye ukufumanisa utshintsho kwinqanaba lokubonakaliswa kwemolekyuli ekujoliswe kuyo ebangelwa yi-aptamer.

(2) Uvavanyo lwenqanaba leseli: Ngokusebenzisa iteknoloji yokukhulisa iiseli, ii-aptamers zifakwa kwiiseli ezijoliswe kuzo ukuze zijonge utshintsho lwebhayoloji olufana nokwakheka kweeseli, ukwanda kwazo kunye ne-apoptosis, kwaye zivavanye umsebenzi webhayoloji wee-aptamers.

(3) Uvavanyo lwemodeli yezilwanyana: Kwiimodeli zezilwanyana ezifanelekileyo, ii-aptamers ze-nucleic acid zinikwa ngenaliti okanye ngokunikezelwa kwamayeza, kwaye izalathisi zomzimba kunye notshintsho lwe-pathological lwezilwanyana ziyajongwa ukuvavanya umsebenzi webhayoloji kunye nokhuseleko lwee-aptamers emzimbeni.