Iqonga leTekhnoloji yeHybridoma
I-Alpha Lifetech inika abathengi iimveliso kunye neenkonzo ze-antibody eziqinisekisiweyo ezisemgangathweni kunye nee-antibody eziphinda zihlanganiswe.
Cela iKoteshini01
Iqonga leTekhnoloji yeHybridoma
I-Alpha Lifetech inamava amaninzi kwicandelo lophuhliso lwee-antibody. Kwiminyaka edlulileyo, i-Alpha Lifetech iseke iqonga elibanzi lophuhliso lwee-antibody (kuquka uphuhliso lwee-antibody ze-monoclonal, uphuhliso lwee-antibody ze-polyclonal, uphuhliso lwee-antibody ze-monoclonal eziphinda zisetyenziswe, kunye nezinye iinkonzo zokulungiselela nokuphuhlisa ii-antibody).
I-Alpha Lifetech inika abathengi iimveliso kunye neenkonzo ze-antibody eziqinisekisiweyo ezisemgangathweni kunye nee-antibody eziphinda zihlanganiswe, kwaye ii-antibodies ezilungisiweyo zineenkcukacha eziqinileyo kunye nozinzo oluhle. Izixhobo ezininzi zokucoca ii-antibody kunye nezixhobo zingasetyenziselwa ukubonelela ngeenkonzo zokucoca ii-antibody ezivela kwimithombo eyahlukeneyo efana nomvundla, igusha, inkukhu, ii-antibodies ze-monoclonal zegundane, kunye neenkonzo zokucoca i-affinity yeproteni ye-A/G kunye neenkonzo zokwahlula ii-antibody.
Ngokusekelwe kwiindlela ezahlukeneyo zokulungiselela ii-antibody ezifana netekhnoloji ye-hybridoma cell, i-Alpha Lifetech inokubonelela ngeenkonzo ezenzelwe wena kwii-antibody ze-monoclonal kunye ne-polyclonal zeentlobo ezahlukeneyo. Ukongeza, uluhlu lweenkonzo ukusuka kulandelelwano oluphezulu lwe-throughput, ukulungiswa kwee-antibody, ukucocwa kwee-antibody ukuya ekuqinisekisweni kwee-antibody nako kunokunikezelwa. Siza kukhetha indlela yokucoca ngokusekelwe kwinjongo ethile yomthengi. Ngokusekelwe kwiinkqubo zeqonga ezibanzi ezifana neqonga lophuhliso lwee-antibody kunye neqonga leproteni, i-Alpha Lifetech igubungela iinkonzo zokuvelisa ii-antibody ezisezantsi nezisezantsi, inikezela ngokucocwa kwee-antibody, ulandelelwano oluphezulu lwe-antibody, ukuqinisekiswa kwee-antibody kunye nezinye iinkonzo zobugcisa.
Ubuchwepheshe beHybridoma
Itekhnoloji yeHybridomma ingasetyenziselwa ukulungiselela ii-antibodies ze-monoclonal. Ngokudibanisa iiseli ze-spleen ezivela kwiimpuku ezigonyiweyo kunye neeseli ze-myeloma ezivela ebantwini okanye kwiimpuku, iiseli ze-hybridoma zenziwa kwaye zikhuphe ii-antibodies ezithile. Ii-antibodies ze-monoclonal eziveliswe yimpuku zingasetyenziswa kuphando lwesayensi olufana ne-enzyme-linked immunosorbent assay, i-radioimmunoassay, i-immunohistochemistry, i-flow cytometry, i-protein purification, njl. njl. Iisampuli ze-antibody ze-Monoclonal ziquka i-ascites, i-hybridoma supernatant, njl. Isampuli efunyenwe ngokufaka iiseli ze-hybridoma kwisisu sezilwanyana kunye nokusebenzisa indawo ye-physiological yesisu sesilwanyana ukuvelisa ii-antibodies ibizwa ngokuba yi-ascites sample. Iiseli zeHybridom ezifakwe kwisisu sezilwanyana zinokukhula kwaye zikhuphe ii-antibodies ze-monoclonal, ezinokuqokelelana kwi-ascites. Ngenxa yokuba kunokwenzeka ukuba kubekho ukungcola (iiproteni zezilwanyana, iihomoni, njl.njl.) kwezi antibodies, kufuneka ukucoca. Ngokusekelwe kumsebenzi wokukhupha ii-B lymphocytes, ii-antibodies ze-monoclonal zihlala zikhutshwa kwindawo yokukhulisa xa kukhuliswa iiseli ze-hybridoma. Ulwelo olune-monoclonal antibodies kwindawo yokukhulisa imfuyo lubizwa ngokuba yi-hybridoma supernatant. Ubunyulu bee-monoclonal antibodies kwi-supernatant buphezulu kakhulu, kodwa kufuneka ukucoca ngakumbi ukuze kulungiselelwe ngobuninzi njengoko zifunyanwa nge-in vitro culture.
Ukucocwa kwe-antibody kunokwahlula ii-antibodies ezicocekileyo kakhulu kwii-complexes. Okwangoku, ukucocwa kwe-antibody affinity kunye nokuhlukaniswa kwe-antibody kunye nokucocwa zizinto ezisetyenziswa kakhulu ekucocweni kwe-antibody, ikakhulu kubandakanya ukucocwa kwe-protein A/G, i-ion exchange chromatography, i-gel filtration, i-precipitation kunye ne-hydrophobic interaction chromatography. Phakathi kwazo, inkonzo yokucocwa kwe-protein A/G affinity ithandwa kakhulu kwizicelo ezahlukeneyo zokucocwa kwe-antibody. Inkqubo ye-Alpha Lifetech yokulungiselela ii-antibodies ze-monoclonal ezisekelwe kwitekhnoloji ye-hybridoma iboniswe kuMfanekiso 1.
Umzobo 1 Umzobo wenkqubo yokulungiselela ii-antibodies ze-monoclonal ezisekelwe kwitekhnoloji ye-hybridoma.
I-Antibody Drug Conjugate kunye neTekhnoloji yeHybridoma
I-Antibody drug conjugate (ADC) sisixhobo esitsha sebhayoloji esidibanisa ii-antibodies ngeekhemikhali kunye namayeza e-cytotoxic. I-Antibody drug conjugate idibanisa amandla athile okuqaphela ii-antibodies kunye nesiphumo esinamandla sokubulala amachiza e-cytotoxic, kwaye ihambisa ngokuchanekileyo amayeza e-cytotoxic kwiiseli ze-tumor ngokusebenzisa ii-antibodies, ngaloo ndlela ifezekisa unyango oluchanekileyo. Inxalenye ye-antibody kumayeza e-ADC idla ngokulungiswa ngetekhnoloji ye-hybridoma okanye ezinye iindlela zokuvelisa ii-antibody, ezifana netekhnoloji ye-genetic engineering antibody. Itekhnoloji ye-Hybridoma ibonelela ngezinto eziluhlaza ze-antibody ezikumgangatho ophezulu zamayeza e-ADC. Amayeza e-ADC kunye nee-antibodies ze-monoclonal ezilungiselelwe ngetekhnoloji ye-hybridoma zidlala indima ebalulekileyo kwiinkalo ezifana nonyango lomhlaza. Zingasetyenziswa zodwa okanye zidityaniswe ukuphucula ukusebenza kakuhle konyango.
Ii-antibodies ze-monoclonal ezilungiselelwe yiteknoloji ye-hybridoma zinobungcali obuphezulu, zinokubopha ngokuchanekileyo kwaye zibone ii-antigen ezithile, kwaye zinokuveliswa ngomlinganiselo omkhulu. Ngokusebenzisa iteknoloji yokulandelelanisa i-hybridoma, ii-antibody genes kwiiseli ze-hybridoma zinokukhutshwa kwaye zilandelelaniswe ukuze kufunyanwe ulandelelwano oluhambelanayo, oluluncedo ekubonakalisweni kwe-antibody okulandelayo. Ii-antibodies ze-monoclonal ezilungiselelwe ngeteknoloji ye-hybridoma zingasetyenziselwa ukufumanisa iimpawu ezithile zesifo, ezifana neempawu ze-tumor, ii-antigen ze-viral, njl.njl., zibonelela ngenkxaso enamandla yokuxilongwa kwangethuba kwezifo. Ukongeza, ingasetyenziswa nakwizilingo ezifana ne-immunoblotting kunye ne-immunohistochemistry ukufunda ukubonakaliswa kweprotheni, indawo, ulwakhiwo, kunye nomsebenzi.
Itekhnoloji ye-hybridoma Imveliso yeNkonzo ye-Antibody
| Amanyathelo | Umxholo weNkonzo | Umgca wexesha |
|---|---|---|
| Inyathelo 1: Ukugonywa kwezilwanyana | (1) Ukugonywa kwezilwanyana izihlandlo ezi-4, ukugonywa kwe-booster idosi e-1, iyonke iidosi ezi-5 zigonyiwe. (2) I-serum engalunganga ngaphambi kokuba kugonywe, kwaye i-ELISA yenziwe kwidosi yesine ukuze kufunyanwe i-serum titer. (3) Ukuba i-serum antibody titer yedosi yesine iyahlangabezana neemfuno, idosi enye eyongezelelweyo yokugonywa iya kunikwa kwiintsuku ezi-7 ngaphambi kokuqokelelwa kwegazi. Ukuba ayihlangabezani neemfuno, ukugonywa rhoqo kuya kuqhubeka. (4) Amandla afanelekileyo, iiseli ze-spleen ziyahlukaniswa ukuze kudityaniswe iiseli. | Iiveki ezili-10-12 |
| Inyathelo lesi-2: Ukudibanisa kunye nokuhlola | (1) Ukuhlanganiswa: Emva kokugonywa, iiseli zespleen yempuku zaqokelelwa zaza zadityaniswa neeseli ze-myeloma. (2) Ukwanda nokuhlolwa: Iiseli ze-hybridoma ezifunyenweyo zanda kwaye zahlolwa i-ELISA ukuze kufunyanwe ii-clones ezisebenzayo kakhulu. | Iiveki ezi-6 |
| Inyathelo lesi-3: I-Subclone | Ukwahlula iiseli zomama ezili-15-25 ezintle kusetyenziswa indlela yokunyibilikisa elinganiselweyo, kwaye ekugqibeleni kuchongwe ii-clones ezili-10-15 ezintle | Iiveki ezi-3-4 |
| Inyathelo lesi-4: Ukuveliswa kwe-Ascites kunye nokuCoca i-Antibody | (1) Ukuveliswa kwee-Ascites: Iintlobo ezili-10-15 ze-hybridoma zisetyenziselwa ukuvelisa ii-ascites kwiimpuku (ukuba kufunyenwe inani elikhulu leentlobo ze-hybridoma, ziya kuthunyelwa zikwimo yeseli) (2) Ukuchongwa kwe-Ascites: Ukufunyanwa kwe-Ascites ELISA, amandla (iproteni/intsholongwane>10 ^ 5, imolekyuli encinci/ipeptide>10 ^ 4) yokucoca umlambo osezantsi (3) Ukucocwa kweProtheyini A/G: Ukucocwa kweAscites | Iiveki ezi-3-4 |
| Inyathelo lesi-5: Ukuhanjiswa | (1) Nika iintlobo ezi-3-5 ze-monoclonal antibody hybridoma. (2) Iseli nganye ye-hybridoma inika i-1mg ye-antibody ecocekileyo (ukuhlanjululwa kwe-ascites). (3) Ingxelo yeNkqubo yoVavanyo | Iveki e-1 |
Iintlobo ezininzi
Ukhuselo lomzimba lunokubonelelwa kwizilwanyana ezahlukeneyo, kuquka kodwa kungaphelelanga apho kwiimpuku, imivundla, iigusha, njl.
Iithagethi ezininzi
Ngokusekelwe kwitekhnoloji ye-hybridoma, iinkonzo ezininzi zokufumanisa ii-antibody ezijoliswe kuzo ziyafumaneka: iiproteni, iipeptide, iimolekyuli ezincinci, iintsholongwane, iiproteni ze-membrane, i-mRNA, njl.njl.
Iirekhodi zokulandelelwa
Imigangatho yokulawula umgangatho we-QC (amandla omzimba, ulawulo lomgangatho we-PBMC, ulawulo lomgangatho wethala leencwadi, kunye nolawulo lomgangatho wokuqinisekisa ukuhlolwa), iingxelo zovavanyo zesiNgesi, iirekhodi zovavanyo zokuqala
Inkqubo Eyenzelwe Wena
Ukwenziwa ngokwezifiso kwenkqubo yomntu ngamnye ukuze kuhlangatyezwane neemfuno zeprojekthi yophando zabathengi abahlukeneyo
Inkonzo yoPhuhliso lwee-Antibody zeMonoclonal
Singabonelela ngeenkonzo zophuhliso lwee-antibody ze-monoclonal ezikumgangatho ophezulu, ezicocekileyo kakhulu, nezicacileyo kakhulu, kubandakanya ukuveliswa kwee-antibodies ze-monoclonal zegundane kunye nee-antibodies ze-rabbit monoclonal.
Iqonga leTekhnoloji yeHybridoma
Kubandakanya inkqubo yokugonya, iinkonzo zokulungiselela i-antibody, ukucocwa kwe-antibody, ukulandelelana kwe-antibody ephezulu, ukuqinisekiswa kwe-antibody, njl.
Iqonga lokuHlela iiSeli ze-B enye
I-Alpha Lifetech ineengenelo ngexesha lokuhlola kunye nokufumana ii-antibodies ezisemgangathweni ophezulu. Ingabonelela ngoyilo lwe-antigen, ukwenziwa, kunye nokuguqulwa, ukukhuselwa kwezilwanyana, ukuhlolwa kokutyetyiswa kweseli enye ye-B, kunye nokulandelelana kweseli enye.
Iqonga loPhuhliso lwe-Antibody Display yePhage
I-Alpha Lifetech inokubonelela ngeenkonzo zobugcisa zophuhliso lwee-antibody zokubonisa i-phage ukususela ekulungiseleleni ii-antibody, ukucocwa kwee-antibody, ukulandelelana kwee-antibody, njl.
Izixhobo zobuchwephesha ezinxulumene noko
Intshayelelo kwiTekhnoloji yeHybridomaIVIDIYO
Ukuba unemibuzo, nceda ukhululeke ukunxibelelana nathi nangaliphi na ixesha.
Leave Your Message
2018-07-16 
0102