Inkonzo yokuCoca iiProteyini zeMembrane

I-Alpha Lifetech ilungiselele ngempumelelo amawaka eeproteni eziphinda-phindayo kubathengi bethu, kuquka ii-antibodies eziphinda-phindayo, iiproteni ezikhutshweyo, iiproteni ze-transmembrane, iiprotease, njl.njl., ngamanqanaba aphezulu empumelelo. Abathengi badinga kuphela ukubonelela ngolandelelwano lweproteni, igama le-CDS okanye leproteni, kwaye i-Alpha Lifetech inokuphuhlisa isicwangciso esipheleleyo sokudibanisa iiproteni kunye nokucoca ngexesha elifutshane. Singabonelela ngeenkonzo zokubonakalisa iiproteni ngokwezifiso ngokusekelwe kwiinkqubo ezine eziphambili zokubonakalisa, kwaye sikhethe amaqhinga ahlukeneyo okudibanisa iiproteni kunye nokucoca ngokweemfuno zabathengi.

I-Alpha Lifetech inezixhobo ezahlukeneyo zokucoca iiproteni, ezinokubonelela ngeenkonzo zokucoca iiproteni zendalo kunye neenkonzo zokucoca iiproteni eziphinda zihlanganiswe, ezifana nokucoca i-affinity, i-molecular sieve chromatography, i-ion exchange chromatography, kunye ne-hydrophobic chromatography. Siza kukhetha iindlela zokucoca ngokweemfuno ezithile zabathengi bethu, size sizidibanise neqonga lethu lokucoca iiproteni ukuze sifumane iimveliso zeeproteni ezisemgangathweni ngexesha elifutshane.

Ulwahlulo lweeProteni zeMembrane

Ngokwendlela iiproteni ezisasazwa ngayo kwi-membrane kunye nobudlelwane phakathi kweeproteni ze-membrane kunye ne-membrane lipids, iiproteni ze-membrane zahlulwe zibe ziindidi ezintathu, kuquka iiproteni ze-peripheral membrane, iiproteni ze-membrane ezihlanganisiweyo, kunye neeproteni ezixhasa i-lipid.

Iproteni yesiteshi se-ionic

Iprotheyini yesiteshi se-Ionic yiprotheyini yeseli edibeneyo efakwa kwiiseli zebhayoloji ukuze zenze izakhiwo ezithile zesiteshi, ngokukhetha ivumela ii-ion ezithile ukuba zithuthwe kwiiseli ngaphakathi nangaphandle kweseli, ngelixa zithintela ezinye ii-ion ukuba zingadluli. Ukukhetha iiprotheyini zesiteshi se-ion kubalulekile ekugcineni imisebenzi yobomi beseli efana nomahluko kuxinzelelo lwee-ion phakathi kweseli nangaphandle kwayo, ukuthuthwa kwee-neurotransmitter, njl.

Ii-receptors ezidityanisiweyo zeproteni ye-G

Ii-receptors ezidityanisiweyo zeproteni ye-G ligama eliqhelekileyo leklasi enkulu yee-receptors zeproteni ye-membrane, ezizezona zininzi zee-receptors zomphezulu weseli kwi-eukaryotes. Umsebenzi ophambili wee-receptors ezidityanisiweyo zeproteni ye-G kukudlulisela ulwazi olungaphandle kweseli ngaphakathi kweeseli ngokusebenzisana neproteni ye-G, edlala indima ebalulekileyo ekudlulisweni kwesignali. Ii-receptors ezidityanisiweyo zeproteni ye-G ziyaqaphela kwaye zibopha kwi-ligands ezingaphandle kweseli ukuze ziqalise iindlela zokubonisana ngaphakathi kweseli, ngaloo ndlela zilawula imisebenzi eyahlukeneyo ye-physiological yeeseli.

Iinxalenye ezifana neentsholongwane

Isuntswana elifana nentsholongwane lisuntswana leproteni elenziwe ngokuzihlanganisa kweproteni enye okanye ezingaphezulu zesakhiwo sentsholongwane. Ezi suntswana zifana ngokwemo kunye nokwakheka kweesuntswana zentsholongwane zendalo, kodwa azinazo izinto zemfuza zentsholongwane, ngoko ke azisuleli.

IiNgcambu ezifana neeVirus zingasebenza njengeqonga lokubonisa iiproteni zemembrane, zilinganisa ukwakheka kunye nomsebenzi weeproteni zemembrane kwiindawo zendalo ngokubonisa iiproteni ezithile zemembrane kwiVirus Like Particles. Ekuphuhlisweni kwegonyo, ii-antigens ezikhuselayo zentsholongwane, ngokuqhelekileyo iiproteni zemembrane, zinokuboniswa kwiVirus Like Particles ukukhuthaza umzimba ukuba uvelise iimpendulo zomzimba ngokuchasene nezi antigens. Iigonyo ezilungiselelwe ngale ndlela zikhuselekile kakhulu kwaye zivuselela umzimba.

Ukucocwa kweeProteni zeMembrane

Kukho iindlela ezahlukeneyo zokucoca iiproteni ze-membrane, apho i-affinity chromatography isebenzisa i-affinity yeeproteni ze-membrane ngokwazo okanye ii-fusion tag, ezifana nee-His tag, kunye ne-ligands zazo ezithile ukufezekisa ukucocwa kunye nokwahlulwa kweproteni ye-membrane. I-ion exchange chromatography isebenzisa iipropati zokutshaja zeeproteni ze-membrane ukuze zahlule kwi-ion exchange resins. Nangona kunjalo, izisombululo zeproteni ye-membrane zihlala ziqulathe izicoci, kwaye ukurhoxa kunokuchaphazela ukusebenza kwe-ion exchange chromatography. I-gel filtration chromatography kukusebenzisa umahluko wobunzima be-membrane protein molecular ukuze zahlule kwikholamu ye-gel, ngale ndlela, sinokufumana i-high purity membrane proteins. Ngenxa ye-affinity kunye ne-hydrophobicity yeeproteni ze-membrane, zinokwahlulwa kwiikholamu ze-chromatographic. Le ndlela iyasebenza, iyakhawuleza, kwaye inovelwano kakhulu, kodwa ixabiso likwaphezulu.

ukucocwa kweproteni ye-membrane-Alpha Lifetech

Umzobo 1 Umzobo oyintloko wokucocwa kwe-protein affinity

Iingenelo zokucocwa kweproteni ye-membraneKUTHENI UKUBA Ukhethe

Ukulungiswa kweCodon

Ukubonakaliswa kweprotheyini enyibilikayo kakhulu kunye nomsebenzi weprotheyini ephezulu kunokuqinisekiswa

Iindlela zokubilisa iiproteni ezininzi: ezincinci (1L, 10L, kunye ne-30L), ezinkulu (80L, 130L, 250L, kunye neetanki zokubilisa ze-500L)

Ukulungiswa kweeproteni eziphinda-phindayo ezicocekileyo kakhulu kwiimiligram kunye neegram ngexesha elifutshane

I-endotoxin ephantsi: Indlela yokufumanisa i-LAL

Inkqubo epheleleyo yokuxhasa uxwebhu lwe-GMP iqinisekisa ukulandeleka kwazo zonke izixhobo, ii-reagents, kunye nolwazi lokulungiselela imveliso.

Buyela kwiPhepha lePlatform yeMembrane Protein

Ukuba unemibuzo, nceda ukhululeke ukunxibelelana nathi nangaliphi na ixesha.

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