IQonga lokuPhuhlisa iQonga lokuBonisa i-Antibody

I-Alpha Lifetech, enamava ayo abanzi eprojekthi ekufumaneni i-antibody, inokubonelela ngeenkonzo ze-monoclonal kunye ne-polyclonal antibody kwiintlobo ezininzi, kunye nokwakhiwa kwethala leencwadi kunye neenkonzo zokuhlola. I-Alpha Lifetech inokubonelela abathengi nge-antibody eqinisekisiweyo esemgangathweni kunye neemveliso ze-antibody ezihlangeneyo, kunye neenkonzo ezihambelanayo, ukulungiselela ii-antibodies ezisebenzayo, ezicacileyo kakhulu, kunye nezizinzileyo. Ngokusebenzisa amaqonga e-antibody abanzi kunye nobunjineli be-antibody, sibonelela ngeenkonzo ezigubungela umsinga onyukayo kunye nosezantsi kwemveliso ye-antibody, kubandakanywa neenkonzo zobuchwepheshe ezifana nokulungiselela isilwa-mntu, ukucocwa, ukulandelelana kwe-antibody, kunye nokuqinisekiswa.

I-Alpha Lifetech ineqonga lokufumanisa i-antibody eliqolileyo elibonelela abathengi ngoluhlu lweenkonzo ezibandakanya ukugonywa kwezilwanyana, ukwakhiwa kwethala leencwadi le-phage kunye nokuhlolwa, ukulandelelana kwe-antibody, ukubonakaliswa kwe-antibody, ukuhlanjululwa kwe-antibody, ukuqinisekiswa kwe-antibody, kunye nokubhalwa kwe-antibody ngokusekelwe kwi-teknoloji ye-hybridoma, iteknoloji ye-cell B enye, iteknoloji yokubonisa i-phage, kunye nokunye. I-Alpha Lifetech inomgca wemveliso osemgangathweni, kwaye ukongeza kwiinkonzo zokulungiselela i-antibody, iinkonzo ezixhasayo ezifana ne-antibody humanization, i-antibody affinity maturation, i-ADC yoyilo lwechiza kunye nophuhliso, kunye noyilo olulandelayo lwe-CAR-T lunokubonelelwa. Ngelo xesha, i-Alpha Lifetech iye yakha ilayibrari ye-antibody esekelwe kwi-M13, i-T4, kunye ne-T7 i-platform ye-phage yokubonisa i-phage, kunye nomthamo wokugcina ukuya kwi-10 ^ 8-10 ^ 9. Izinga elincomekayo, izinga lofakelo, kunye nokwahluka kwethala leencwadi konke kunokufikelela ngaphezu kwama-90%.

Phage ukubonisa iTekhnoloji

Ukuveliswa kwee-antibodies ze-Monoclonal ekuqaleni kwalungiswa kusetyenziswa iteknoloji ye-hybridoma monoclonal antibody ukuvelisa i-mouse hybridoma monoclonal antibody. Ngokukodwa, ngokudibanisa iiseli ze-spleen ezivela kwiimpuku ezigonyiweyo kunye neeseli ze-myeloma ezivela ebantwini okanye kwiigundane, iiseli ze-hybridoma zenziwe, ezikhupha i-hybridoma monoclonal antibody antibodies. Ngokuguqulwa komntu kwi-mouse monoclonal antibodies kunye nokuguqulwa kofuzo lobunjineli be-antibodies ukubanika ummandla osisigxina we-immunoglobulin yomntu, ukugonywa kwabo kungancitshiswa. Ubunjineli be-Monoclonal antibody busetyenziswa ngokubanzi kwi-biomedical kunye nezicelo zeklinikhi.

I-Alpha Lifetech inokubonelela ngeenkonzo zobunjineli zokuzikhusela kwi-antibody kwindawo enye ukusuka kuyilo lweprothokholi yophando lwangaphambi kweklinikhi ukuya kwi-antibody drug conjugates (ADCs) ukufunyanwa kunye nokuqinisekiswa kwezilwanyana. I-antibody drug conjugates (ADCs) inokuhambisa amachiza echemotherapy kwiiseli zomhlaza. Emva kokuzibophelela kwiithagethi ezithile ezichazwe kwiiseli zomhlaza, i-ADC ikhupha amachiza e-cytotoxic kwiiseli zomhlaza. I-Alpha Lifetech inokubonelela abathengi ngeenkonzo zokuvuthwa kwe-antibody. Ngokuphuculwa koguqulo oluphezulu kunye neteknoloji yokuhlola i-phage ephezulu, izilwa-buhlungu ezinobudlelwane obuthile zijongwa kuqala, kwaye emva koko iinguqu ze-amino acid ziyaziswa ukuvelisa iintlobo ezahlukeneyo. Emva koko, ii-antibodies eziphezulu zobudlelwane zavavanywa kwaye zahlolwa kusetyenziswa iindlela zokuhlola. Emva kwemijikelo emininzi yokwenziwa ngcono kunye nohlalutyo lwesakhiwo, izilwa-buhlungu ezinobudlelwane obuphezulu kunye nobugqwirha obuqinileyo ekugqibeleni zafunyanwa.

I-Alpha Lifetech inokwakha iindidi ezahlukeneyo zethala leencwadi lokubonisa i-antibody phage, kubandakanywa amathala eencwadi omzimba, amathala eencwadi emveli, amathala eencwadi angama-synthetic, kunye namathala eencwadi okwenziwa. Ngokusekwe kumthamo ophezulu weelayibrari ze-antibody, izilwa-buhlungu ezikhethekileyo zinokufunyanwa. Iivectors ze-phagemid ezininzi ezifana ne-pMECS, pComb3X, kunye ne-pCANTAB 5E zinokubonelelwa, kunye neentlobo ezifana ne-TG1 E. coli, i-XL1 Blue, kunye ne-ER2738. Ukongeza kumthamo wethala leencwadi ukuya kuthi ga kwi-10^9, izinga lokufakwa kwamaqhekeza ekujoliswe kulo liphezulu, lidala iimeko ezaneleyo zokuhlola izilwa-buhlungu ezanelisayo abathengi. I-flowchart yenkonzo yokulungiselela i-antibody ye-Alpha Lifetech esekelwe kubugcisa bokubonisa i-phage iboniswe kuMfanekiso woku-1.

Umzobo.1 Umzobo wenkqubo yokulungiselela i-antibodies ye-monoclonal esekelwe kwi-teknoloji yokubonisa i-phage.

Phage Display Antibody Production Workflow

Amanyathelo	Umxholo weNkonzo	Umgca wexesha
Inyathelo 1: Ugonyo lwezilwanyana	(1) Ukugonywa kwezilwanyana ngamaxesha e-4, ukugonywa kwe-booster 1 idosi, i-5 iyonke iidosi ezigonyiweyo. (2) I-serum engalunganga ngaphambi kokuba kugonywe, kwaye i-ELISA yenziwa kwi-dose yesine ukufumana i-serum titer. (3) Ukuba i-serum antibody titer yedosi yesine iyahlangabezana neemfuno, idosi enye eyongezelelweyo yogonyo iya kunikezelwa kwiintsuku ezisi-7 phambi kokuqokelelwa kwegazi. Ukuba ngaba ayizifezekisi iimfuno, ugonyo lwesiqhelo luya kuqhubeka. (4) Amandla afanelekileyo, ukuqokelela kwegazi kunye nokuhlukana kwee-monocytes	Iiveki ezili-10
Inyathelo 2: ULungiselelo lwe-cDNA	(1) PBMC Iyonke iRNA Extraction (i-RNA Extraction Kit) (2) Ukunyaniseka okuphezulu kwe-RT-PCR ukulungiswa kwe-cDNA (ikhithi yokukhuphela umva)	usuku olu-1
Inyathelo lesi-3: Ukwakhiwa kweThala leeNcwadi le-Antibody	(1) Ukusebenzisa i-cDNA njenge template, iijini zandiswa ngemijikelo emibini yePCR. (2) Ukwakhiwa kwePhage kunye nokuguqulwa: i-gene splicing phagemid vector, ukuguqulwa kwe-electroporation ye-TG1 i-bacteria host, ukwakhiwa kwelayibrari ye-antibody. 3 (4) Uncedo lokulungiswa kwephage: I-M13 phage amplification + purification. (5) Ukuhlangulwa kwethala leencwadi kwiphage	Iiveki ezi-3-4
INyathelo 4: Uvavanyo lweThala leeNcwadi le-Antibody (imijikelo emi-3)	(1) Ukuhlolwa okungagqibekanga kwe-3-rounding (ukuhlolwa kwesigaba esiqinileyo): ukuhlolwa koxinzelelo lokususa ii-antibodies ezingekho ngqo kwinqanaba eliphezulu. (2) I-Clone eyodwa yokukhulisa i-bacteriophage ekhethiweyo+I-IPTG i-induced expression+ELISA ukufunyanwa kwee-clones ezilungileyo. (3) Zonke ii-clones ezilungileyo zikhethwe ngokulandelelana kwemfuza.	Iiveki ezi-4-5

I-advanteji yeTekhnoloji yokubonisa i-phage

I-Alpha Lifetech inamava atyebileyo kwicandelo lophuhliso lwe-antibody. Ukutyhubela iminyaka, i-Alpha Lifetech iye yaseka iqonga lophuhliso lolwahlulo olubanzi.

Iinkonzo zeNkxaso

Sinokubonelela ngeenkonzo ezahlukeneyo zolwakhiwo lwethala leencwadi lokuzikhusela kwizilwanyana kunye neenkonzo zovavanyo lwethala leencwadi lendalo le-antibody ngokweemfuno zabathengi

Iinjongo ezininzi

Iinkonzo ezininzi zokufunyanwa kwe-antibody ziyafumaneka: iiproteni, iipeptides, iimolekyuli ezincinci, iintsholongwane, iiproteni zemembrane, i-mRNA, njl.

IiVektha ezininzi

Inkonzo yolwakhiwo lwethala leencwadi lobuqu, sinokubonelela ngeevektha ezahlukeneyo ze-bacteriophage kubandakanya i-PMECS, i-pComb3X, kunye ne-pCANTAB 5E, kwaye siyiguqule ngokweemfuno zabathengi.

Iqonga labaqolileyo

Umthamo wokugcina unokufikelela kwi-10 ^ 8-10 ^ 9, amazinga okufakwa angaphezulu kwe-90%, kunye nobudlelwane be-antibodies obufunyenwe ngokuhlolwa ngokubanzi kwinqanaba le-nM pM.

INKONZO enxulumeneyo

Ubuchule boPhuhliso lwe-Antibody Multiple

Inkonzo yoPhuhliso lwe-Monoclonal Antibody

I-Hybridoma Technology Platform

IQonga lokuhlela iSeli eNye B

IQonga lokuPhuhlisa iQonga lokuBonisa i-Antibody

uphuhliso lwe-antibody monoclonal-Alpha Lifetech

Inkonzo yoPhuhliso lwe-Monoclonal Antibody

Sinokubonelela ngeenkonzo ezikumgangatho ophezulu, ezicocekileyo, kunye neenkonzo ezithile zophuhliso lwe-monoclonal antibody, kubandakanywa nokuveliswa kwe-mouse monoclonal antibodies kunye nomvundla we-monoclonal antibodies.

I-Hybridoma Technology Platform

Kubandakanya inkqubo yogonyo, iinkonzo zokulungiselela i-antibody, ukucocwa kwe-antibody, ulandelelwano lwe-antibody ephezulu, ukuqinisekiswa kwe-antibody, njl.

IQonga lokuhlela iSeli eNye B

I-Alpha Lifetech ineenzuzo kwixesha lokuhlola kunye nokufumana amajoni omzimba akumgangatho ophezulu. Inokubonelela ngoyilo lwe-antigen, i-synthesis, kunye nokuguqulwa, ukunganyangeki kwezilwanyana, ukuhlolwa kwe-cell eyodwa ye-B yokutyebisa, ukulandelelana kweseli enye.