Isevisi Yokuhlaziya Izimpawu ze-Aptamer

I-Alpha Lifetech ibilokhu inikeza izinsizakalo ezihlobene ne-aptamer iminyaka eminingi, futhi okwamanje ingahlinzeka ngezinhlelo eziphelele zokuthuthukisa i-aptamer ezifana nokuhlanganiswa kwe-aptamer, ukuhlolwa kwe-SELEX, ukulandelana okuphezulu, kanye nokwenza ngcono kanye nokuchaza i-aptamer. Ngokusekelwe ezinzuzweni zendlela ye-SELEX yokuhlola ama-aptamer, i-Alpha Lifetech yandise izindlela eziningi zokuhlola ama-aptamer.

Isingeniso Sokuhlaziywa Kwezimpawu ze-Aptamer

Ukuqinisekiswa kobudlelwano

I-aptamer affinity elungiselelwe kahle iqinisekiswe ngamasu afanele okuhlolwa kokubopha kwe-aptamer. Izibonelo zifaka phakathi i-Isothermal Titration Calorimetry (ITC), i-Flow Cytometry (FCM), kanye ne-Surface Plasmon Resonance (SPR), i-Microfluidics, njll. I-Aptamer affinity ivame ukuvezwa yi-dissociation constant (KD), inani elibonakalayo elisetshenziselwa ukulinganisa izinga lokuhlukaniswa kwama-molecule ekusabeleni okuguqukayo. Uma inani le-KD lincane, i-complex izinze kakhulu, okungukuthi, i-affinity iqine kakhulu; Ngokuphambene nalokho, lapho inani le-KD lilikhulu, i-complex ingazinzi kakhulu futhi i-affinity iba buthakathaka. Lesi sinyathelo siyisihluthulelo sokubona ukusebenza kwe-aptamer, ukuqinisekisa ukuvuthwa kwe-aptamer affinity futhi singabopha i-molecule eqondiwe nge-affinity ephezulu nokukhetha ezinhlelweni ezisebenzayo.

Umfanekiso 1 Inqubo yokuvuthwa kwe-aptamer affinity. Umthombo wokubhekisela:IKinghorn AB, eFraser LA, 2017.

Ukuqinisekiswa komsebenzi

Ngaphezu kokuqinisekiswa kwe-affinity, umsebenzi we-aptamer udinga ukuqinisekiswa. Lokhu kuhlanganisa ukuqinisekisa ukuqina, ukucaca, kanye nokusebenzisana kwe-aptamer namanye ama-molecule endaweni ethile. Imiphumela yokuqinisekiswa kokusebenza izothinta ngqo inani lama-aptamer ocwaningweni lwesayensi kanye nezicelo zezimboni.

Ukuqinisekiswa Okuqondile

Ukuhlolwa okuncintisanayo: Amandla e-aptamer okubopha esihlokweni esithile ahlolwa lapho kukhona amanye ama-targets afanayo. Uma i-aptamer ingabopha ngqo e-molecule esihlokweni ngaphandle kokuphazamiseka kwamanye ama-molecule, kubonisa ukuthi inobungcweti obuphezulu.

Ukuhlolwa kokusabela okuphambene: I-aptamer iboshelwe ochungechungeni lwezinhloso ezihlobene noma ezingahlobene ukuze ibone ukuthi iboshelwe kuphela kuhloso ethile, ukuze kuqinisekiswe ukucaca kwayo.

Ukuqinisekiswa kokuzinza

Ukuhlolwa kokuwohloka kwe-nuclease: Ama-aptamer atholakale ekugxilweni okuhlukene kwama-nuclease futhi ukuwohloka kwawo kwabonwa. Ngokuqhathanisa izinga lokuwohloka ngezikhathi ezahlukene, ikhono lokulwa nokuwohloka kwe-nuclease le-aptamer lingahlolwa.

Ukuhlolwa kokuqina kwezinga lokushisa nesikhathi: I-aptamer ibekwe ngaphansi kwezimo zokushisa nesikhathi ezahlukene ukuze kubonwe ukuqina kwayo kwesakhiwo nokusebenza kwayo. Lokhu kusiza ekunqumeni izimo zokugcina nokusetshenziswa ezifanele kakhulu zama-aptamer.

Ukuhlonza umsebenzi webhayoloji

Indlela efanele ivame ukukhethwa ngokuya ngomgomo othize wokusetshenziswa kwe-nucleic acid aptamer.

(1) Ukuhlolwa kwezinga lama-molecule: Ukusebenzisa amasu e-molecular biology, njenge-gel electrophoresis, i-Western blot, njll., ukuthola ama-complexes akhiwe ngemva kokuba i-aptamer ihlangana ne-molecule eqondiwe, noma ukuthola izinguquko ezingeni lokubonakaliswa kwe-molecule eqondiwe ebangelwa yi-aptamer.

(2) Ukuhlolwa kwezinga lamaseli: Ngokusebenzisa ubuchwepheshe bokukhulisa amaseli, ama-aptamer afakwa amaseli aqondiwe ukuze abone izinguquko zezinto eziphilayo ezifana nokwakheka kwamaseli, ukwanda kwawo kanye ne-apoptosis, futhi ahlole umsebenzi wezinto eziphilayo wama-aptamer.

(3) Ukuhlolwa kwemodeli yezilwane: Kumamodeli ezilwane afanele, ama-aptamer e-nucleic acid anikezwa ngomjovo noma ngokuphathwa kwemithi, futhi izinkomba zomzimba kanye nezinguquko ze-pathological zezilwane ziyabhekwa ukuze kuhlolwe umsebenzi webhayoloji kanye nokuphepha kwama-aptamer emzimbeni.