I-Aptamer Development Platform

Ama-Aptamers ayi-oligonucleotide enomucu owodwa (i-DNA, i-RNA noma i-XNA) enendawo yokuhlobana okuphezulu kanye nokucaciswa okuphezulu okubopha ngokuqondile ama-molecule afana namasosha omzimba, futhi asetshenziselwa kakhulu ukuthuthukiswa kwezinzwa, ukuxilongwa kanye nokwelapha.

Inkundla ye-aptamer ehlinzekwe yi-Alpha Lifetech ihlanganisa izigaba ezimbili: iplathifomu ye-aptamer synthesis, ebandakanya ikakhulukazi isevisi ye-SELEX aptamer library synthesis kanye nensizakalo yokuthuthukisa i-aptamer (DNA, RNA noma i-XNA), kanye nesiteji sokuhlola i-aptamer okuhlanganisa nezinsizakalo zokuhlola ezisekelwe kubuchwepheshe be-SELEX bamaprotheni, ama-peptide, amaseli, ama-molecule amancane, ama-molecule aqondiswe phansi njenge-amertamer ions kanye nokunye. izinsiza zokuhlaziya ukuhlonza.

I-Aptamer Synthesis Platform

Isevisi ye-SELEX aptamer synthesis library

Isevisi yokuhlanganisa umtapo wezincwadi we-SELEX aptamer ikakhulukazi ihilela ukwakha umtapo wolwazi oqukethe inani elikhulu lokulandelana kwe-oligonucleotide enomucu owodwa nge-in vitro chemical synthesis ngokuya ngamamolekyuli okuqondiwe. Ukwakhiwa komtapo wolwazi kuyisiqalo sobuchwepheshe be-SELEX, obuhlinzeka ngokulandelana okuningi kwamakhandidethi enqubweni elandelayo yokuhlola ngokwakha imitapo yolwazi emikhulu engahleliwe futhi kwandise amathuba okuhlola ama-aptamers ahambisana kakhulu.

I-Library synthesis ihlukaniswe kakhulu ngezinyathelo ezilandelayo:

Izinyathelo	Imininingwane Yezobuchwepheshe
Khomba Ama-molekyuli Okuhlosiwe	Khomba amangqamuzana okuqondiwe adinga ukuhlolelwa ama-aptamers, okungaba amaprotheni, ama-nucleic acid, amangqamuzana amancane, ama-ion ensimbi, njll.
Idizayini yokulandelana okungahleliwe	Ubude bokulandelana okungahleliwe, ukwakheka kwesisekelo kanye neminye imingcele kwaklanywa ngokuvumelana nezici zama-molecule aqondiwe kanye nezidingo zokuhlola. Ngokuvamile, ukulandelana okungahleliwe kuphakathi kwamashumi namakhulu ezisekelo ngobude.
Ukuhlanganiswa Kokulandelana Okugxilile	Izingcezu ze-oligonucleotide ezinokulandelana okungaguquki (okufana nokulandelana kweprimer ye-PCR) kuzo zombili iziphetho zenzelwe futhi zahlanganiswa, ezizosetshenziswa enqubweni elandelayo yokukhulisa nokuhlola.

Umtapo wolwazi ohlanganisiwe usadinga ukuqhutshekiselwa phambili ukucubungula ukuze kulawulwe ikhwalithi. Ukugxila komtapo wolwazi kwanqunywa ukuthi kuqinisekiswe ukusebenza kwawo enqubweni yokuhlolwa elandelayo. Ukuhlukahluka nokunemba kokulandelana okungahleliwe kumtapo wolwazi kwaqinisekiswa ngokulandelana nangezinye izindlela zokuqinisekisa ukuthi ikhwalithi yomtapo wolwazi ihlangabezana nezidingo zokuhlolwa.

Ngalezi zinyathelo ezingenhla, umtapo wezincwadi we-SELEX wekhwalithi ephezulu nohluke kakhulu ungahlanganiswa, onganikeza ukulandelana kwamakhandidethi okuchichimayo kwenqubo yokuhlola elandelayo.

Izinsizakalo zokuthuthukisa i-Aptamer (i-DNA, i-RNA noma i-XNA)

Ama-aptamers ngokuvamile abhekisela kuma-nucleic acid aptamers. Ama-aptamers e-Nucleic acid afaka ama-aptamers e-DNA, ama-RNA aptamers, nama-XNA aptamers aguqulwa ngamakhemikhali e-nucleic acid. Indlela ye-SELEX isetshenziswa kabanzi ekuthuthukisweni kwama-aptamers. Ukugeleza komsebenzi okuyisisekelo kwezinsizakalo zokuthuthukiswa kwe-aptamer kufaka phakathi ukwakhiwa komtapo wolwazi, ukubophezela okuqondiwe, ukuhlukaniswa nokuhlanza, ukukhulisa, imijikelezo eminingi yokuhlola, kanye nokuhlonza ukulandelana. Iminyaka eminingi, sigxile ekwakhiweni komtapo wolwazi kanye nolwazi olucebile ekuthuthukisweni kwe-aptamer. Sizibophezele ekuhlinzekeni amakhasimende ngezinsizakalo ezingcono.

Inqubo ye-SELEX Technology

Inqubo ye-SELEX iqukethe imizuliswano eminingi, ngayinye enezinyathelo ezibalulekile ezilandelayo:

Ukubophezela Umtapowolwazi kanye Nempokophelo

Umtapo wezincwadi we-nucleic acid owakhiwe uxutshwe nama-molecule athile okuhlosiwe (njengamaprotheni, inhlanganisela ye-molecule encane, njll.), ukuze ukulandelana kwe-nucleic acid emtatsheni wezincwadi kube nethuba lokuhlanganisa ama-molecule okuhlosiwe.

Ukuhlukaniswa kwama-Molecule Angaboshiwe

Ukulandelana kwe-Nucleic acid okungaboshiwe ku-molecule eqondiwe kuhlukaniswa nengxube ngezindlela ezithile ezifana ne-affinity chromatography, ukuhlukaniswa kobuhlalu obuzibuthe, njll.

Ukukhuliswa kwama-Moleculi Abophezelayo

Ukulandelana kwe-nucleic acid okuboshelwe ku-molecule eqondiwe kuyakhuliswa, ngokuvamile kusetshenziswa ubuchwepheshe be-polymerase chain reaction (PCR). Esigabeni esilandelayo sokuhlola, ukulandelana okukhulisiwe kuzosetshenziswa njengomtapo wolwazi wokuqala.

Umfanekiso 1: Inqubo yokuhlola ye-SELEX

I-Aptamer Screening Platform

Isevisi yokuhlola i-Aptamer

I-Alpha Lifetech inikezela ngezinsizakalo ezahlukahlukene zokuhlola i-aptamer zisebenzisa izindlela ezahlukahlukene ze-SELEX zezinhlobo ezahlukahlukene zama-molecule akho:

Izinhlobo Okuhlosiwe	Imininingwane Yezobuchwepheshe
Ukuhlolwa kwe-Protein Aptamer ngu-SELEX	Injongo eyinhloko yokuhlolwa kwe-protein aptamer ukuhlola ama-aptamers angabopha ngokuqondile ama-molecule amaprotheni. Lawa ma-aptamers kulula ukuwahlanganisa, azinzile futhi angenwa kalula yizinto zemvelo.
Ukuhlolwa kwe-Peptide Aptamer ngu-SELEX	Ama-aptamers e-Peptide ayikilasi lama-peptide amafushane alandelanayo anencazelo ephezulu kanye nokuhlobana, okungabophezela ngokuqondile ezintweni eziqondiwe futhi abonise inhlobonhlobo yamandla okusebenza emkhakheni we-biomedical. Ngenqubo ethile yokuhlola, ama-aptamers e-peptide angabopha ngokuqondile kuma-molecule ayahlolwa kusuka enanini elikhulu lamalabhulali alandelanayo e-peptide.
Ukuhlolwa kwe-Aptamer okuqondene neseli (Cell-SELEX)	Amaseli aqondiwe noma ama-molecule athile endaweni yeseli alungiselelwa njengethagethi. Okuqondiwe kungaba amaseli aphelele, ama-receptors kulwelwesi lweseli, amaprotheni, noma amanye ama-molecule amancane.
Ukuhlolwa kwe-Molecule Aptamer encane nge-Capture SELEX	I-Capture SELEX iyindlela yokuhlola ye-in vitro yokuhlola ama-aptamers ama-molecule amancane, okuhlukile kwe-SELEX. I-Capture SELEX ifaneleka kahle ekuhlolweni kwe-aptamer kokuhlosiwe kwe-molecule encane, ngokuvamile enamaqembu ambalwa asebenzayo futhi okunzima ukuwafaka ngokuqondile ekusekelweni kwesigaba esiqinile.
Izinsizakalo ze-SELEX ezisekelwe ezilwaneni ezibukhoma	Isevisi yokuhlola esekelwe ezilwaneni ebukhoma iyindlela yokuhlola esetshenziswa kabanzi emkhakheni wesayensi yezinto eziphilayo, imithi kanye ne-biotechnology, esebenzisa izilwane eziphilayo njengamamodeli okuhlola ukuhlola nokuhlola ama-molecule athile, udokotela, ukwelashwa noma izinqubo zebhayoloji. Amasevisi aklanyelwe ukulingisa indawo yokuphila emzimbeni womuntu ukuze abikezele ngokunembe kakhudlwana futhi ahlole ukusebenza kahle nokuphepha kwemiphumela yokuhlolwa emzimbeni womuntu.

Isevisi yokwenza i-Aptamer

I-hydrophilicity, ukulahleka kokuhlobana okuphezulu ngesikhathi sokukhiqiza, kanye nokuphuma ngokushesha kwama-aptamers kunciphisa ukusetshenziswa kwawo. Njengamanje, kuhlolwe izindlela ezahlukahlukene zokuthuthukisa ukuze kuthuthukiswe ukusebenza kwama-aptamers.

Futhi sinezindlela ezihlukahlukene zokuthuthukisa i-aptamer ehlanganisa ukunqunywa, ukuguqulwa, ukuhlanganisa eqenjini elifanele (thiol, carboxy, amine, fluorophore, njll.).

Isevisi yokuhlaziya izici ze-Aptamer

Isevisi yokuhlaziya izici ze-Aptamer ibhekisela kusevisi yobungcweti yokulungiswa kwesakhiwo sokuhlolwa kokusebenza kanye nokuqinisekiswa kokusebenza kwe-aptamer etholiwe ukuze kuqinisekiswe ukuthi i-aptamer ihlangabezana nekhono elithile lokubopha, ukuqina kanye nezidingo ezicacile. Ihlanganisa kakhulukazi ukuhlaziya ukuhlobana kanye nokucacisa, ukuhlola ukuzinza nokuqinisekiswa komsebenzi webhayoloji.