Isevisi Yokuhlanza Amaprotheni E-Membrane

I-Alpha Lifetech ilungiselele ngempumelelo izinkulungwane zamaprotheni ahlanganisiwe kumakhasimende ethu, okuhlanganisa ama-antibodies ahlanganisiwe, amaprotheni akhishwe, amaprotheni e-transmembrane, ama-protease, njll., ngamazinga aphezulu empumelelo. Amakhasimende adinga kuphela ukunikeza ukulandelana kwamaprotheni, igama le-CDS noma le-protein, futhi i-Alpha Lifetech ingakha uhlelo oluphelele lokubonakaliswa kwamaprotheni ahlanganisiwe kanye nokuhlanza ngesikhathi esifushane. Singahlinzeka ngezinsizakalo zokuvezwa kwamaprotheni ezenziwe ngokwezifiso ngokusekelwe ezinhlelweni ezine ezinkulu zokuvezwa, futhi sikhethe amasu ahlukene okuvezwa kwamaprotheni ahlanganisiwe kanye nokuhlanza ngokwezidingo zamakhasimende.

I-Alpha Lifetech inezinhlobo ezahlukene zemishini yokuhlanza amaprotheni, engahlinzeka ngezinsizakalo zokuhlanza amaprotheni zemvelo kanye nezinsizakalo zokuhlanza amaprotheni aphinde ahlanganiswe, njengokuhlanzwa kwe-affinity, i-molecular sieve chromatography, i-ion exchange chromatography, kanye ne-hydrophobic chromatography. Sizokhetha izindlela zokuhlanza ngokwezidingo ezithile zamakhasimende ethu, futhi sizihlanganise nepulatifomu yethu yokuhlanza amaprotheni ukuze sithole imikhiqizo yamaprotheni esezingeni eliphezulu ngesikhathi esifushane.

Ukuhlukaniswa Kwamaprotheni E-Membrane

Ngokusho kokusatshalaliswa kwamaprotheni kulwelwesi kanye nobudlelwano phakathi kwamaprotheni elwelwesi kanye nama-lipid elwelwesi, amaprotheni elwelwesi ahlukaniswe ngezigaba ezintathu, okuhlanganisa amaprotheni elwelwesi olungaphandle, amaprotheni elwelwesi oluhlanganisiwe, kanye namaprotheni asekela i-lipid.

Iphrotheni yesiteshi se-Ionic

Iphrotheni yesiteshi se-Ionic iyiphrotheni ye-membrane ehlanganisiwe efakwa kuma-membrane ezinto eziphilayo ukuze yakhe izakhiwo ezithile zesiteshi, ivumela ngokukhetha ama-ion athile ukuthi athuthwe kuwo wonke ama-membrane ngaphakathi nangaphandle kweseli, kuyilapho ivimbela amanye ama-ion ukuthi angadluli. Ukukhetha kwamaprotheni esiteshi se-ion kubalulekile ekugcineni imisebenzi yokuphila kweseli njengomehluko ekugxilweni kwama-ion phakathi kwangaphakathi nangaphandle kweseli, ukuthuthwa kwe-neurotransmitter, njll.

Ama-receptor ahlanganisiwe e-G protein

Ama-receptor ahlanganisiwe e-G protein ayigama elijwayelekile lesigaba esikhulu sama-receptor e-membrane protein, okuyiwona ama-receptor amaningi kakhulu ebusweni bamaseli kuma-eukaryotes. Umsebenzi oyinhloko wama-receptor ahlanganisiwe e-G protein ukudlulisa ulwazi olungaphandle kweseli ngaphakathi kwamaseli ngokusebenzisana ne-G protein, edlala indima ebalulekile ekudlulisweni kwesignali. Ama-receptor ahlanganisiwe e-G protein aqaphela futhi abopha kuma-ligand angaphandle kweseli ukuze aqalise izindlela zokubonisana zangaphakathi kweseli, ngaleyo ndlela alawule imisebenzi eyahlukahlukene yomzimba yamaseli.

Izinhlayiya Ezifana Negciwane

Inhlayiya efana negciwane iyinhlayiya yephrotheni eyakhiwa ukuzihlanganisa kwephrotheni eyodwa noma ngaphezulu yesakhiwo segciwane. Lezi zinhlayiya zifana ngesimo kanye nesakhiwo sezinhlayiya zemvelo zegciwane, kodwa azinazo izakhi zofuzo zegciwane, ngakho-ke azithelelani.

Izinhlayiya Ezifana Nezigciwane zingasebenza njengeplatifomu yokubonisa amaprotheni e-membrane, zilingisa ukwakheka kanye nomsebenzi wamaprotheni e-membrane ezindaweni zemvelo ngokubonisa amaprotheni athile e-membrane ku-Virus Like Particles. Ekuthuthukisweni kwemithi yokugoma, ama-antigen avikelayo egciwane, ngokuvamile amaprotheni e-membrane, angaboniswa ku-Virus Like Particles ukuze kukhuthazwe umzimba ukuthi ukhiqize izimpendulo zomzimba ngokumelene nala ma-antigen. Imijovo elungiselelwe ngale ndlela iphephile kakhulu futhi ayivimbeli umzimba.

Ukuhlanzwa Kwamaprotheni E-Membrane

Kunezindlela ezahlukahlukene zokuhlanza amaprotheni e-membrane, phakathi kwazo i-affinity chromatography isebenzisa i-affinity yamaprotheni e-membrane ngokwawo noma ama-fusion tag, njenge-His tag, nama-ligands awo athile ukuze kufezwe ukuhlanzwa nokuhlukaniswa kwamaprotheni e-membrane. I-ion exchange chromatography isebenzisa izakhiwo zokushaja zamaprotheni e-membrane ukuze zihlukaniswe kuma-ion exchange resins. Kodwa-ke, izixazululo zamaprotheni e-membrane zivame ukuqukatha izihlanzi, futhi ukwehla kungathinta ukusebenza kwe-ion exchange chromatography. I-gel filtration chromatography iwukusebenzisa umehluko wesisindo sama-molecule e-membrane protein ukuze ihlukaniswe kukholamu ye-gel, ngale ndlela, singathola i-high purity membrane protein. Ngenxa ye-affinity kanye ne-hydrophobicity yamaprotheni e-membrane, angahlukaniswa kumakholomu e-chromatographic. Le ndlela iyasebenza, iyashesha, futhi inozwela kakhulu, kodwa izindleko nazo ziphakeme kakhulu.

ukuhlanzwa kwamaprotheni e-membrane - Alpha Lifetech

Umfanekiso 1 Umdwebo oyinhloko wokuhlanzwa kwe-protein affinity

Izinzuzo zokuhlanzwa kwamaprotheni e-membraneKUNGANI ukhethe

Ukulungiswa kwe-Codon

Ukuvezwa kwamaprotheni amaningi ancibilikayo kanye nomsebenzi wamaprotheni amaningi kungaqinisekiswa

Izindlela zokubilisa amaprotheni ezikali eziningi: ezincane (1L, 10L, kanye no-30L), ezinkulu (80L, 130L, 250L, kanye namathangi okubilisa angu-500L)

Ukulungiswa kwamaprotheni aphinde ahlanganiswe ngobumsulwa obuphezulu ngama-milligram namagremu ngesikhathi esifushane

I-endotoxin ephansi: Indlela yokuthola i-LAL

Uhlelo oluphelele lokusekela amadokhumenti e-GMP luqinisekisa ukulandeleka kwazo zonke izinto, ama-reagent, kanye nolwazi lokulungiselela umkhiqizo.

Buyela ekhasini lePlatform ye-Membrane Protein

Uma unemibuzo, sicela ukhululeke ukuxhumana nathi nganoma yisiphi isikhathi.

Leave Your Message

Isevisi Evelele

0102