Isingeniso Sezincwadi Zokulwa Namagciwane Ezihlanganisiwe

Umtapo wolwazi we-antibody ohlanganisiwe, owaziwa nangokuthi umtapo wolwazi we-De novo, uyindlela yokwenziwa esebenzisa amasu anjengokwenziwa kwe-DNA noma ukubonisa i-phage ukuklama nokuhlanganisa izifunda eziguquguqukayo ze-antibody eziphelele, kufaka phakathi izifunda zohlaka kanye nama-CDR, ngaphandle kokuthembela emitatsheni yolwazi ye-antibody engazi lutho.

Umtapo wolwazi we-antibody okwenziwa kancane kancane udalwa ngokuhlanganisa imitapo yolwazi ye-antibody eyenzeka ngokwemvelo kanye nokwehlukahluka kokwenziwa. Ngokuvamile kuhilela ukuhlanganiswa kwama-oligonucleotide e-DNA ukuze kukhiqizwe isethi yezindawo ezahlukene ezinqumayo ezihambisanayo (ama-CDR), ezihlanganiswa nohlaka lwe-antibody oluhlala lukhona oluvela emithonjeni yemvelo efana namaseli e-B abantu noma ezilwaneni. I-CDR enziwe kancane kancane yethula ukwehlukahluka emtatsheni wolwazi, okuholela kuma-antibodies ahlose izinhlobo eziningi zama-epitopes. Umtapo wolwazi we-antibody okwenziwa kancane kancane unikeza ukuvumelana phakathi kokuhlukahluka kwemvelo kwesistimu yomzimba kanye nokuhlukahluka okulawulwayo okutholakala ngezindlela zokwenziwa.

Umehluko phakathi kwemitapo yolwazi ye-antibody e-naïve kanye ne-synthetic uncike emthonjeni wezakhi zofuzo ze-immunoglobulin. Okwamanje, umehluko phakathi komtapo wolwazi we-antibody owenziwe kancane futhi owenziwe ulele ekutheni owokuqala uqukethe ingxenye yezingxenye ze-antibody e-naïve, njenge-chain elula noma i-heavy chain, futhi eyodwa kuphela yazo ekhiqizwa ku-vitro; kuyilapho leyo ekhiqizwa itholakala ngokuphelele ekuhlanganisweni kokwenziwa yi-PCR ku-vitro.

I-Alpha Lifetech ingahlinzeka

I-Alpha Lifetech Inc.inganikeza umtapo wolwazi we-antibody owenziwe kancane/owenziwe kabusha ovela ezilwaneni nakubantu ngokusekelwe epulatifomu yethu yobungcweti yokuthola ama-antibody. Njengoba uneminyaka eminingi yesipiliyoni ekuguqulweni kwezakhi zofuzo kanye nokwakhiwa komtapo wolwazi, imitapo yolwazi eyenziwe kabusha ingakhiwa ekwazi ukukhiqiza ama-antibody okwenziwa ngokuqina nangokucacile okungaphezu kwamandla ama-antibody emvelo.I-Alpha Lifetech'sochwepheshe bayaziqhenya ngokumemezela ukuthi singaqinisekisa izinga eliphezulu lempumelelo ekwakhiweni komtapo wolwazi we-antibody oqukethe ama-clone azimele angu-10^8 - 10^10.

I-Alpha Lifetech Inc.iyaziqhenya ngokunikeza izinsizakalo zokwakha umtapo wolwazi we-antibody eziphelele ukuze kuhlangatshezwane nezidingo ezahlukahlukene zabacwaningi bomhlaba wonke, okuhlanganisa i-scFv, i-Fab, i-VHH antibody, kanye nemitapo yolwazi eyenziwe ngokwezifiso. Inhloso yethu ukuqonda nokuhlangabezana nezidingo zamakhasimende ahlukene nokusiza nganoma yiziphi izinkinga ezizayo nezivelayo emsebenzini wocwaningo.

Inqubo Yesevisi Yokwakha YamaLabhulali E-Antibody Ahlanganisiwe

Umklamo
Isigaba sokuklama sihilela ukukhetha uhlaka lwe-antibody kanye nezifunda ezinquma ukuhambisana (ama-CDR) ngokusekelwe ku-antibody sequences ekhona noma idatha yesakhiwo. Ama-antibody e-CDR ahlanganisiwe angaklanywa futhi ukwethula imisebenzi ethile noma ukuthuthukisa izakhiwo zokubopha.

Ukwenziwa
I-DNA yokwenziwa ebhala ama-antibody sequences aklanyelwe, okuhlanganisa izifunda zohlaka kanye nama-CDR, yenziwa kusetshenziswa izindlela zamakhemikhali noma ze-enzyme.

Ukuhlanganiswa
Izingcezu ze-DNA ezihlanganisiwe zihlanganiswa zibe amavekhtha okuveza ama-antibody kusetshenziswa amasu anjenge-PCR, i-ligation, noma i-Gibson assembly. Lawa mavekhtha angafakwa ezinhlelweni zokuveza njengamaseli ebhaktheriya, imvubelo, noma ezilwane ezincelisayo ukuze kukhiqizwe ama-antibody.

Ukuhlola Nokukhetha
Imitapo yolwazi yama-antibody eyakhiwe iyahlolwa futhi ikhethwe ama-antibody anezakhiwo ezifiselekayo kusetshenziswa izindlela zokuhlola ezisebenzisa i-high-throughput. Lokhu kungafaka amasu afana nokubonisa i-phage, ukubonisa i-yeast, noma ukubonisa i-ribosome, kuye ngefomethi yomtapo kanye nokusetshenziswa kwawo.

Isevisi Yokwakha Amalabhulali Okuvikela Umzimba Ahlanganisiwe

I-Alpha Lifetech Inc.Ihlinzeka amakhasimende ngomklamo owodwa kanye nensizakalo yokwenziwa kwemitapo yolwazi ye-antibody ngokubopha ama-repertoire e-V-gene aqinile futhi alula abe yi-phage vector isebenzisa uhlelo lwe-Cre-lox lokuhlanganisa indawo ethile ukuze yakhe i-repertoire yama-Fabs aboniswe ku-phage equkethe ama-clone angu-6.5 × 10^10. Umtapo wolwazi uveze i-Abs ngokumelene nama-antigen amaningi, amanye ane-nanomolar affinities. Ukukhiqizwa komtapo wolwazi we-antibody womuntu kusekelwe kubuchwepheshe bokubonisa i-M13 phage (okuboniswe esithombeni esingezansi).